EFCBP2 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-84002
![Immunohistochemistry-Paraffin: EFCBP2 Antibody [NBP1-84002]](https://resources.rndsystems.com/images/products/EFCBP2-Antibody-Immunohistochemistry-Paraffin-NBP1-84002-img0030.jpg "Immunohistochemistry-Paraffin: EFCBP2 Antibody [NBP1-84002]")
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Key Product Details
Validated by
Orthogonal Validation, Independent Antibodies
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Rat (97%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VILDIFRRADKNDDGKLSLEEFQLFFADGVLNEKELEDLFHTIDSDNTNHVDTKELCDYFVDHMGDYEDVLASLETLNHSVLKAMGYTKKVYEGGSNVDQFVTRFLLKETANQIQSLLSSVESAVEAIEEQTSQL
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for EFCBP2 Antibody - BSA Free
![Immunohistochemistry-Paraffin: EFCBP2 Antibody [NBP1-84002]](https://resources.rndsystems.com/images/products/EFCBP2-Antibody-Immunohistochemistry-Paraffin-NBP1-84002-img0026.jpg "Immunohistochemistry-Paraffin: EFCBP2 Antibody [NBP1-84002]")
Immunohistochemistry-Paraffin: EFCBP2 Antibody [NBP1-84002]
Immunohistochemistry-Paraffin: EFCBP2 Antibody [NBP1-84002] - Staining of human cerebral cortex, kidney, pancreas and skin using Anti-NECAB2 antibody NBP1-84002 (A) shows similar protein distribution across tissues to independent antibody NBP1-84001 (B).![Immunocytochemistry/ Immunofluorescence: EFCBP2 Antibody [NBP1-84002]](https://resources.rndsystems.com/images/products/EFCBP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84002-img0032.jpg "Immunocytochemistry/ Immunofluorescence: EFCBP2 Antibody [NBP1-84002]")
Immunocytochemistry/ Immunofluorescence: EFCBP2 Antibody [NBP1-84002]
Immunocytochemistry/Immunofluorescence: EFCBP2 Antibody [NBP1-84002] - Staining of human cell line U-2 OS shows localization to vesicles.![Immunohistochemistry-Paraffin: EFCBP2 Antibody [NBP1-84002]](https://resources.rndsystems.com/images/products/EFCBP2-Antibody-Immunohistochemistry-Paraffin-NBP1-84002-img0031.jpg "Immunohistochemistry-Paraffin: EFCBP2 Antibody [NBP1-84002]")
Immunohistochemistry-Paraffin: EFCBP2 Antibody [NBP1-84002]
Immunohistochemistry-Paraffin: EFCBP2 Antibody [NBP1-84002] - Staining of human kidney shows no positivity in cells in tubules as expected.![Immunohistochemistry: EFCBP2 Antibody [NBP1-84002]](https://resources.rndsystems.com/images/products/EFCBP2-Antibody-Immunohistochemistry-NBP1-84002-img0013.jpg "Immunohistochemistry: EFCBP2 Antibody [NBP1-84002]")
Immunohistochemistry: EFCBP2 Antibody [NBP1-84002]
Immunohistochemistry: EFCBP2 Antibody [NBP1-84002] - Staining mouse hippocampus shows immunoreactivity in a subset of neurons.![Immunohistochemistry: EFCBP2 Antibody [NBP1-84002]](https://resources.rndsystems.com/images/products/EFCBP2-Antibody-Immunohistochemistry-NBP1-84002-img0012.jpg "Immunohistochemistry: EFCBP2 Antibody [NBP1-84002]")
Immunohistochemistry: EFCBP2 Antibody [NBP1-84002]
Immunohistochemistry: EFCBP2 Antibody [NBP1-84002] - Staining mouse caudate putamen shows immunoreactivity in medium spiny neurons and synapses.![Immunohistochemistry: EFCBP2 Antibody [NBP1-84002]](https://resources.rndsystems.com/images/products/EFCBP2-Antibody-Immunohistochemistry-NBP1-84002-img0011.jpg "Immunohistochemistry: EFCBP2 Antibody [NBP1-84002]")
Immunohistochemistry: EFCBP2 Antibody [NBP1-84002]
Immunohistochemistry: EFCBP2 Antibody [NBP1-84002] - Staining mouse facial nucleus shows distinct positivity in neuronal cell bodies and processes.![Immunohistochemistry: EFCBP2 Antibody [NBP1-84002]](https://resources.rndsystems.com/images/products/EFCBP2-Antibody-Immunohistochemistry-NBP1-84002-img0010.jpg "Immunohistochemistry: EFCBP2 Antibody [NBP1-84002]")
Immunohistochemistry: EFCBP2 Antibody [NBP1-84002]
Immunohistochemistry: EFCBP2 Antibody [NBP1-84002] - Staining mouse midbrain shows neuronal positivity in dorsal raphe.![Immunohistochemistry-Paraffin: EFCBP2 Antibody [NBP1-84002]](https://resources.rndsystems.com/images/products/EFCBP2-Antibody-Immunohistochemistry-Paraffin-NBP1-84002-img0025.jpg "Immunohistochemistry-Paraffin: EFCBP2 Antibody [NBP1-84002]")
Immunohistochemistry-Paraffin: EFCBP2 Antibody [NBP1-84002]
Immunohistochemistry-Paraffin: EFCBP2 Antibody [NBP1-84002] - Staining of human cerebral cortex shows moderate positivity in neuropil.![Immunohistochemistry-Paraffin: EFCBP2 Antibody [NBP1-84002]](https://resources.rndsystems.com/images/products/EFCBP2-Antibody-Immunohistochemistry-Paraffin-NBP1-84002-img0028.jpg "Immunohistochemistry-Paraffin: EFCBP2 Antibody [NBP1-84002]")
Immunohistochemistry-Paraffin: EFCBP2 Antibody [NBP1-84002]
Immunohistochemistry-Paraffin: EFCBP2 Antibody [NBP1-84002] - Staining of human pancreas shows weak cytoplasmic positivity in islets of Langerhans.![Immunohistochemistry-Paraffin: EFCBP2 Antibody [NBP1-84002]](https://resources.rndsystems.com/images/products/EFCBP2-Antibody-Immunohistochemistry-Paraffin-NBP1-84002-img0029.jpg "Immunohistochemistry-Paraffin: EFCBP2 Antibody [NBP1-84002]")
Immunohistochemistry-Paraffin: EFCBP2 Antibody [NBP1-84002]
Immunohistochemistry-Paraffin: EFCBP2 Antibody [NBP1-84002] - Staining of human skin shows no positivity in squamous epithelial cells as expected.![EFCBP2 Antibody - BSA Free Western Blot: EFCBP2 Antibody - BSA Free [NBP1-84002]](https://resources.rndsystems.com/images/products/nbp1-84002_rabbit-polyclonal-efcbp2-antibody-842025177543.jpg "Western Blot: EFCBP2 Antibody - BSA Free [NBP1-84002]")
Western Blot: EFCBP2 Antibody - BSA Free [NBP1-84002]
Analysis in human cerebral cortex tissue.![EFCBP2 Antibody - BSA Free Western Blot: EFCBP2 Antibody - BSA Free [NBP1-84002]](https://resources.rndsystems.com/images/products/nbp1-84002_rabbit-polyclonal-efcbp2-antibody-84202517254720.jpg "Western Blot: EFCBP2 Antibody - BSA Free [NBP1-84002]")
Western Blot: EFCBP2 Antibody - BSA Free [NBP1-84002]
Analysis in mouse cerebral cortex tissue.Applications for EFCBP2 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:1000 - 1:2500
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: EFCBP2
Alternate Names
EF-hand calcium binding protein 2, N-terminal EF-hand calcium binding protein 2
Gene Symbol
NECAB2
Additional EFCBP2 Products
Product Documents for EFCBP2 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for EFCBP2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for EFCBP2 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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