Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EFHD1 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human EFHD1. The immunogen is located within the first 50 amino acids of EFHD1. Amino Acid Squence: EPKPEPEPPARAPT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EFHD1 Antibody - BSA Free

EFHD1 Antibody - BSA Free

Western Blot: EFHD1 Antibody - BSA Free [NBP1-77134] -

Western Blot: EFHD1 Antibody - BSA Free [NBP1-77134] - Western blot analysis of EFHD1 in human spleen tissue lysate with EFHD1 antibody at (A) 2 and (B) 4 ug/mL.
EFHD1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: EFHD1 Antibody - BSA Free [NBP1-77134] -

Immunocytochemistry/ Immunofluorescence: EFHD1 Antibody - BSA Free [NBP1-77134] - Immunofluorescence of EFHD1 in Rat Spleen cells with EFHD1 antibody at 20 ug/mL.

Applications for EFHD1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Western Blot

2-4 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EFHD1

EFHD1, also known as Swiprosin-2 or SWS2, is an EF-hand and coiled-coil-containing adaptor protein identified in a subtractive hybridization study using a neuronal cell line established from the cerebellum of an adult p53-null mouse, however further study indicated no difference in normal mice. Its mRNA is widely expressed, with its expression in brain undetectable at embryonic stages, with increasing levels from postnatal to adult development. In situ hybridization showed expression in neurons but not white matter of the cerebellum and cerebrum. EHFD1 is also highly expressed in testes, ovary, and the collecting ducts of the kidney, suggesting that in non-neuronal cells, EFHD1 may be involved in gametogenesis and water-reabsorbtion.

Alternate Names

DKFZp781H0842, EF-hand domain family, member D1, EF-hand domain-containing protein 1, EF-hand domain-containing protein D1, member D1, MST133, MSTP133, Swiprosin-2

Entrez Gene IDs

80303 (Human)

Gene Symbol

EFHD1

UniProt

Q9BUP0 (Human)

Additional EFHD1 Products

Product Documents for EFHD1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for EFHD1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for EFHD1 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for EFHD1 Antibody - BSA Free

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  • Q: I have used your EFHD1 antibody (NBP1-71699) and can detect a band at approximately 150kDa. Have you detected this band as well?

    A: EFHD1 protein is 239 amino acids in length with a molecular weight of 27kDa. That is the band that our antibody is recognizing. We have not observed the 150kDa band that you are referring to and cannot comment on its origin. You should observe a band at approximately 27kDa on your Western blot.

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