![Western Blot: EFHD2 Antibody [NB100-1477]](https://resources.rndsystems.com/images/products/EFHD2-Antibody-Western-Blot-NB100-1477-img0014.jpg "Western Blot: EFHD2 Antibody [NB100-1477]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Predicted:
Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence CAAFKELQSTFK corresponding to C-Terminus according to NP_077305.2.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 23959172).
Specificity
This antibody does not cross-react with Mouse Swiprosin 2. Data kindly provided by Sebastian Duetting and Dirk Mielenz.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for EFHD2 Antibody
Western Blot: EFHD2 Antibody [NB100-1477]
Western Blot: EFHD2 Antibody [NB100-1477] - Staining of Human Thymus, Human Lymph node and Human Tonsil lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.![Immunohistochemistry-Paraffin: EFHD2 Antibody [NB100-1477]](https://resources.rndsystems.com/images/products/EFHD2-Antibody-Immunohistochemistry-Paraffin-NB100-1477-img0012.jpg "Immunohistochemistry-Paraffin: EFHD2 Antibody [NB100-1477]")
Immunohistochemistry-Paraffin: EFHD2 Antibody [NB100-1477]
Immunohistochemistry-Paraffin: EFHD2 Antibody [NB100-1477] - Analysis of Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.Applications for EFHD2 Antibody
Application
Recommended Usage
Immunohistochemistry
2 - 4 ug/ml
Immunohistochemistry-Paraffin
2 - 4 ug/ml
Peptide ELISA
Detection limit 1:128000
Western Blot
0.3 - 10 ug/ml
Application Notes
WB: Approx. 30 kDa band observed in human tonsil, thymus and lymph node and in mouse thymus and lymph node lysates (calculated MW of 26.7 kDa band according to NP_077305.2). IHC-P: Human tonsil shows membranous staining in selected cells.
Reviewed Applications
Read 1 review rated 5 using NB100-1477 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: EFHD2
Alternate Names
EF hand domain containing 2, EF hand domain family, member D2, EF-hand domain family, member D2, EF-hand domain-containing protein D2, MGC4342, swiprosin 1, swiprosin-1, SWS1
Gene Symbol
EFHD2
UniProt
Additional EFHD2 Products
Product Documents for EFHD2 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for EFHD2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for EFHD2 Antibody
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Customer Reviews for EFHD2 Antibody (1)
5 out of 5
1 Customer Rating
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Application: Western BlotSample Tested: miceSpecies: miceVerified Customer | Posted 04/27/2017
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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