Eg5 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NB500-181

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Drosophila, Mammal, Plant

Cited:

Human, Mouse, Porcine, Insect - Drosophila, Plant

Applications

Validated:

Western Blot, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Eg5 Primary Antibodies »

Product Specifications

Immunogen

A recombinant segment of the coiled-coil domain of human Eg5 [Uniprot: P52732]

Reactivity Notes

Predicted to react with most mammalian species. Drosophila reactivity reported in scientific literature (PMID: 23888285). Mouse reactivity reported in scientific literature (PMID: 27117404). Plant reactivity reported in scientific literature (PMID: 19527496).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Eg5 Antibody - Azide and BSA Free

Western Blot: Eg5 Antibody [NB500-181]

Western Blot: Eg5 Antibody [NB500-181]

Western Blot: Eg5 Antibody [NB500-181] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY KIF11 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Eg5.
Immunocytochemistry/ Immunofluorescence: Eg5 Antibody [NB500-181]

Immunocytochemistry/ Immunofluorescence: Eg5 Antibody [NB500-181]

Immunocytochemistry/Immunofluorescence: Eg5 Antibody [NB500-181] - Staining of HeLa cells fixed in 3.5% paraformaldehyde. Cytoplasmic staining during interphase and spindle staining during mitosis.
Western Blot: Eg5 Antibody [NB500-181]

Western Blot: Eg5 Antibody [NB500-181]

Eg5-Antibody-Western-Blot-NB500-181-img0006.jpg
Eg5 Antibody

Immunocytochemistry/ Immunofluorescence: Eg5 Antibody [NB500-181] -

Immunocytochemistry/ Immunofluorescence: Eg5 Antibody [NB500-181] - Spontaneous assembly of rosettes in iChM5 derivatives.Feeder free cultures iChM5Ap15 & iChM5Bp28 cells in chamber slides were immunostained as indicated. Left panel, emerging rosettes among self-renewing iPSCs; grayscale inset shows 1x magnification of immunostaining of Oct4 alone. Middle panel shows Nanog staining alone with inset at 2X magnification showing presumptive centrosomes (arrows). Bottom left panel shows forming rosettes immunopositive for Sox2 & Eg5. Bottom right panel shows low magnification image of immunostaining of Sox2 in this iChM5Ap15 culture, showing that virtually all cells were immunopositive. Grey scale inset shows representative forming rosette. Asterisks (*) in each panel indicates example of forming rosette. Scale bar, 50 microns. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25426336), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Eg5 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunoblotting

reported in scientific literature (PMID 28392145)

Immunocytochemistry/ Immunofluorescence

1:1000

Immunoprecipitation

1:10-1:500

Western Blot

1:1000
Application Notes
A band at ~125 kDa can be detected by Western blot.

Reviewed Applications

Read 2 reviews rated 2.5 using NB500-181 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Eg5

Eg5 is a mitotic motor protein required for bipolar spindle formation. The mitotic spindle is crucial for chromosome segregation during cell division.

Long Name

Kinesin-like protein KIF11

Alternate Names

KIF11, KNSL1, TRIP-5, TRIP5

Entrez Gene IDs

3832 (Human); 16551 (Mouse); 171304 (Rat)

Gene Symbol

KIF11

UniProt

P52732 (Human)

Additional Eg5 Products

Product Documents for Eg5 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Eg5 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Eg5 Antibody - Azide and BSA Free

Customer Reviews for Eg5 Antibody - Azide and BSA Free (2)

2.5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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1 Stars
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  • Name: Emily Miller
    Application: Western Blot
    Sample Tested: HeLa, Mouse brain
    Species: Human
    Verified Customer | Posted 04/28/2014
    Control and a knockdown sample probed with Eg5
    Eg5 Antibody - Azide and BSA Free NB500-181
  • Name: Kristin Werner
    Application: Western Blot
    Sample Tested: Mouse tissues, MiaPaCa2, BxPC3, T3M4
    Species: Human
    Verified Customer | Posted 12/16/2013
    Eg5 Antibody - Azide and BSA Free NB500-181

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