EGR1 Antibody (6E8) - Azide and BSA Free

Novus Biologicals | Catalog # H00001958-M03

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Rabbit

Cited:

Mouse, Rabbit

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 6E8

Format

Azide and BSA Free
View all EGR1 Primary Antibodies »

Product Specifications

Immunogen

EGR1 (NP_001955, 444 a.a. ~ 543 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SPVATSYPSPVTTSYPSPATTSYPSPVPTSFSSPGSSTYPSPVHSGFPSPSVATTYSSVPPAFPAQVSSFPSSAVTNSFSASTGLSDMTATFSPRTIEIC

Reactivity Notes

Rabbit reactivity reported in scientific literature.

Specificity

EGR1 - early growth response 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for EGR1 Antibody (6E8) - Azide and BSA Free

Western Blot: EGR1 Antibody (6E8) [H00001958-M03]

Western Blot: EGR1 Antibody (6E8) [H00001958-M03]

Western Blot: EGR1 Antibody (6E8) [H00001958-M03] - EGR1 monoclonal antibody (M03), clone 6E8. Western Blot analysis of EGR1 expression in PC-12.
Immunocytochemistry/ Immunofluorescence: EGR1 Antibody (6E8) [H00001958-M03]

Immunocytochemistry/ Immunofluorescence: EGR1 Antibody (6E8) [H00001958-M03]

Immunocytochemistry/Immunofluorescence: EGR1 Antibody (6E8) [H00001958-M03] - Analysis of monoclonal antibody to EGR1 on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: EGR1 Antibody (6E8) [H00001958-M03]

Immunohistochemistry-Paraffin: EGR1 Antibody (6E8) [H00001958-M03]

Immunohistochemistry-Paraffin: EGR1 Antibody (6E8) [H00001958-M03] - Analysis of monoclonal antibody to EGR1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml
Western Blot: EGR1 Antibody (6E8) [H00001958-M03]

Western Blot: EGR1 Antibody (6E8) [H00001958-M03]

Western Blot: EGR1 Antibody (6E8) [H00001958-M03] - Analysis of EGR1 expression in transfected 293T cell line by EGR1 monoclonal antibody (M03), clone 6E8. Lane 1: EGR1 transfected lysatE (59.73 KDa). Lane 2: Non-transfected lysate.
Western Blot: EGR1 Antibody (6E8) [H00001958-M03]

Western Blot: EGR1 Antibody (6E8) [H00001958-M03]

Western Blot: EGR1 Antibody (6E8) [H00001958-M03] - EGR1 monoclonal antibody (M03), clone 6E8. Western Blot analysis of EGR1 expression in Raw 264.7.
ELISA: EGR1 Antibody (6E8) [H00001958-M03]

ELISA: EGR1 Antibody (6E8) [H00001958-M03]

ELISA: EGR1 Antibody (6E8) [H00001958-M03] - Detection limit for recombinant GST tagged EGR1 is approximately 0.1ng/ml as a capture antibody.

Applications for EGR1 Antibody (6E8) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EGR1

The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene. [provided by RefSeq]

Long Name

Early Growth Response 1

Alternate Names

KROX24, NGFIA, ZNF225

Entrez Gene IDs

1958 (Human)

Gene Symbol

EGR1

OMIM

128990 (Human)

UniProt

NP_001955 (Human)

Additional EGR1 Products

Product Documents for EGR1 Antibody (6E8) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for EGR1 Antibody (6E8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for EGR1 Antibody (6E8) - Azide and BSA Free

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Protocols

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FAQs

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