EGR2 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-92327

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EGR2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of the human EGR2 protein (between amino acids 370-420) [UniProt P11161].

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EGR2 Antibody - BSA Free

Western Blot: EGR2 AntibodyBSA Free [NB100-92327]

Western Blot: EGR2 AntibodyBSA Free [NB100-92327]

Western Blot: EGR2 Antibody [NB100-92327] - Analysis of pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:NIH-3T3 whole cell lysate(40ug) Lane3:Jurkat whole cell lysate(40ug)
Immunocytochemistry/ Immunofluorescence: EGR2 Antibody - BSA Free [NB100-92327]

Immunocytochemistry/ Immunofluorescence: EGR2 Antibody - BSA Free [NB100-92327]

Immunocytochemistry/Immunofluorescence: EGR2 Antibody [NB100-92327] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-EGR2 at 5ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: EGR2 Antibody - BSA Free [NB100-92327]

Immunohistochemistry-Paraffin: EGR2 Antibody - BSA Free [NB100-92327]

Immunohistochemistry-Paraffin: EGR2 Antibody [NB100-92327] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse brain using EGR2 antibody at 1:200 dilution. The signal was detected using HRP-conjugated anti-rabbit secondary antibody and DAB based detection reagent, and the sections were further counterstained with hematoxylin. This EGR2 antibody generated primarily a nuclear staining signal in the neurons and the glial cells, however, some cytoplasmic staining was also observed. The cytoplasmic signal potentially represents the translational or 14-3-3sigma interacting cytoplasmic pool of EGR2.
Western Blot: EGR2 AntibodyBSA Free [NB100-92327]

Western Blot: EGR2 AntibodyBSA Free [NB100-92327]

Western Blot: EGR2 Antibody [NB100-92327] - Egr-2 Antibody in extracts from HUVEC cells.
EGR2 Antibody - BSA Free

ERG2 in Hep G2 Human Cell Line.

ERG2 was detected in immersion fixed Hep G2 human hepatocellular carcinoma cell line using Rabbit anti-ERG2 Affinity Purified Polyclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NB100-92327AF647) (light blue) at 10 µg/mL overnight at 4C. Cells were stained counterstained with DAPI (blue). Cells were imaged using a 40X objective.

Applications for EGR2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 - 10 ug/ml

Immunohistochemistry

2 - 5 ug/ml

Immunohistochemistry-Paraffin

2 - 5 ug/ml

Western Blot

1 - 2 ug/ml
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 1 using NB100-92327 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EGR2

Egr proteins function in transcription regulatory activities surrounding cellular growth, differentiation and function. The deduced amino acid sequences of human Egr-2 and mouse Egr-1 are 92% identical in the zinc finger region but show no homology elsewhere. Egr-2 is a sequence-specific DNA-binding transcription factor that binds two specific DNA sites located in the promoter region of HoxA4 and localizes to the nucleus. Defects in the Egr-2 protein are a cause of congenital hypomyelination neuropathy (CHN). CHN is characterized clinically by early onset of hypotonia, areflexia, distal muscle weakness and very slow nerve conduction velocities. Mutations in the gene that encodes Egr-2 (EGR2) also cause of Dejerine-Sottas syndrome (DSS), which is also known as Dejerine-Sottas neuropathy (DSN) or hereditary motor and sensory neuropathy III (HMSN3). DSS patients exhibit severe early onset motor and sensory neuropathy with very slow nerve conduction velocities and elevated cerebrospinal fluid protein concentrations.

Alternate Names

AT591, CMT1D, CMT4E, early growth response 2, early growth response 2 (Krox-20 homolog, Drosophila), early growth response protein 2, EGR-2, FLJ14547, Krox-20 homolog, Drosophila, KROX-20, Drosophila, homolog (early growth response-2), KROX20DKFZp686J1957, Zinc finger protein Krox-20

Entrez Gene IDs

1959 (Human); 114090 (Rat)

Gene Symbol

EGR2

UniProt

P11161 (Human)

Additional EGR2 Products

Product Documents for EGR2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for EGR2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for EGR2 Antibody - BSA Free

Customer Reviews for EGR2 Antibody - BSA Free (1)

1 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
100%

Have you used EGR2 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • EGR2 Antibody
    Name: Susumu Tasaki
    Application: Immunohistochemistry-Frozen
    Sample Tested: Frozen mouse sciatic nerves and Frozen mouse sciatic nerve
    Species: Mouse
    Verified Customer | Posted 12/05/2019
    Image 1. The staining image using NB100-92327. Image 2. The staining image using NB110-59723 (Previously worked well). Image 3. The staining image of Negative control for NB100-92327.
    EGR2 Antibody - BSA Free NB100-92327
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for EGR2 Antibody - BSA Free

Showing  1 - 1 of 1 FAQ Showing All

  • Q: We would like to try this antibody for staining in Xenopus. The mentioned immunogenic region shows high sequence identity with Xenopus EGR2. Would it be possible to obtain the exact epitope to predict cross reactivity?

    A: I have now been informed that the immunogen sequence for this antibody corresponds to the amino acid range 391-440 of P11161. This shows high homology to the Xenopus sequence, so it is likely that the antibody would bind to Xenopus protein.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies