EHD1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-56035

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EHD1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: MEQPGTAASPVSGSMFSWVSKDARRKKEPELFQTVAEGLRQLYAQKLLP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EHD1 Antibody - BSA Free

Western Blot: EHD1 Antibody [NBP2-56035]

Western Blot: EHD1 Antibody [NBP2-56035]

Western Blot: EHD1 Antibody [NBP2-56035] - Analysis in human cell line A-431 and human cell line HEK 293.
Immunocytochemistry/ Immunofluorescence: EHD1 Antibody [NBP2-56035]

Immunocytochemistry/ Immunofluorescence: EHD1 Antibody [NBP2-56035]

Immunocytochemistry/Immunofluorescence: EHD1 Antibody [NBP2-56035] - Staining of human cell line U-2 OS shows localization to plasma membrane. Antibody staining is shown in green.

Applications for EHD1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. Knockout validation (PMID: 31988382).

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EHD1

The EHD1 gene belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be a

Alternate Names

EH-domain containing 1

Gene Symbol

EHD1

Additional EHD1 Products

Product Documents for EHD1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for EHD1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for EHD1 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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