eIF2 alpha/EIF2S1 Antibody (3H12-C11) - Azide and BSA Free

Novus Biologicals | Catalog # H00001965-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3H12-C11

Format

Azide and BSA Free
View all eIF2 alpha Primary Antibodies »

Product Specifications

Immunogen

EIF2S1 (AAH02513.1, 1 a.a. ~ 315 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPGLSCRFYQHKFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEGMILLSELSRRRIRSINKLIRIGRNECVVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKFTKSKTVYSILRHVAEVLEYTKDEQLESLFQRTAWVFDDKYKRPGYGAYDAFKHAVSDPSILDSLDLNEDEREVLINNINRRLTPQAVKIRADIEVACYGYEGIDAVKEALRAGLNCSTENMPIKINLIAPPRYVMTTTTLERTEGLSVLSQAMAVIKEKIEEKRGVFNVQMEPKVVTDTDETELARQMERLERENAEVDGDDDAEEMEAKAED

Specificity

EIF2S1 - eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for eIF2 alpha/EIF2S1 Antibody (3H12-C11) - Azide and BSA Free

Western Blot: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01]

Western Blot: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01]

Western Blot: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01] - Analysis of EIF2S1 expression in transfected 293T cell line by EIF2S1 monoclonal antibody (M01), clone 3H12-C11.Lane 1: EIF2S1 transfected lysate(36.1 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01]

Immunocytochemistry/ Immunofluorescence: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01]

Immunocytochemistry/Immunofluorescence: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01] - Analysis of monoclonal antibody to EIF2S1 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01]

Western Blot: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01]

Western Blot: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01] - Analysis of EIF2S1 over-expressed 293 cell line, cotransfected with EIF2S1 Validated Chimera RNAi ( Cat # H00001965-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with EIF2S1 monoclonal antibody (M01), clone 3H12-C11 (Cat # H00001965-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01]

Western Blot: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01]

Western Blot: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01] - EIF2S1 monoclonal antibody (M01), clone 3H12-C11 Analysis of EIF2S1 expression in HeLa.
Western Blot: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01]

Western Blot: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01]

Western Blot: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01] - EIF2S1 monoclonal antibody (M01), clone 3H12-C11. Analysis of EIF2S1 expression in Hela S3 NE.
ELISA: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01]

ELISA: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01]

ELISA: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01] - Detection limit for recombinant GST tagged EIF2S1 is approximately 0.3ng/ml as a capture antibody.

Applications for eIF2 alpha/EIF2S1 Antibody (3H12-C11) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: eIF2 alpha

EIF2S1, also known as eIF2a, is the alpha subunit of the translation initiation factor eIF2 complex which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. The phosphorylation state of eIF2S1 controls the rate of tRNA translation. When eIF2a is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2S1 is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation. Recombinant human EIF2S1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Eukaryotic Translation Initiation Factor 2 Alpha

Alternate Names

EIF2S1

Entrez Gene IDs

1965 (Human)

Gene Symbol

EIF2S1

OMIM

603907 (Human)

UniProt

AAH02513 (Human)

Additional eIF2 alpha Products

Product Documents for eIF2 alpha/EIF2S1 Antibody (3H12-C11) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for eIF2 alpha/EIF2S1 Antibody (3H12-C11) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Translation Regulation

Citations for eIF2 alpha/EIF2S1 Antibody (3H12-C11) - Azide and BSA Free

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Protocols

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FAQs

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