eIF2A Antibody (3A7B11) - BSA Free
Novus Biologicals | Catalog # NBP2-26296
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 3A7B11
Format
BSA Free
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Product Specifications
Immunogen
Partial recombinant human EIF2A (between residues 350-585) expressed in E. coli [Uniprot Q9BY44]
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for eIF2A Antibody (3A7B11) - BSA Free
![Western Blot: eIF2A Antibody (3A7B11) [NBP2-26296]](https://resources.rndsystems.com/images/products/eIF2A-Antibody-3A7B11-Western-Blot-NBP2-26296-img0003.jpg "Western Blot: eIF2A Antibody (3A7B11) [NBP2-26296]")
Western Blot: eIF2A Antibody (3A7B11) [NBP2-26296]
Western Blot: eIF2A Antibody (3A7B11) [NBP2-26296] - Western blot analysis of HeLa (1), NIH3T3 (2), A431 (3), MCF-7 (4), Ntera2 (5), PC12 (6), HepG2 (7), and Cos7 (8) cell lysate using eIF2A antibody at a concentration of 2 ug/ml.![Immunocytochemistry/ Immunofluorescence: eIF2A Antibody (3A7B11) [NBP2-26296]](https://resources.rndsystems.com/images/products/eIF2A-Antibody-3A7B11-Immunocytochemistry-Immunofluorescence-NBP2-26296-img0001.jpg "Immunocytochemistry/ Immunofluorescence: eIF2A Antibody (3A7B11) [NBP2-26296]")
Immunocytochemistry/ Immunofluorescence: eIF2A Antibody (3A7B11) [NBP2-26296]
Immunocytochemistry/Immunofluorescence: eIF2A Antibody (3A7B11) [NBP2-26296] - eIF2A antibody (1:50) was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.![Immunohistochemistry-Paraffin: eIF2A Antibody (3A7B11) [NBP2-26296]](https://resources.rndsystems.com/images/products/eIF2A-Antibody-3A7B11-Immunohistochemistry-Paraffin-NBP2-26296-img0005.jpg "Immunohistochemistry-Paraffin: eIF2A Antibody (3A7B11) [NBP2-26296]")
Immunohistochemistry-Paraffin: eIF2A Antibody (3A7B11) [NBP2-26296]
Immunohistochemistry-Paraffin: eIF2A Antibody (3A7B11) [NBP2-26296] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human breast cancer using eIF2A (3A7B11) antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic and perinuclear staining was observed. Staining was performed by Histowiz.Applications for eIF2A Antibody (3A7B11) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:200 - 1:400
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:200
Western Blot
1:500 - 1:2000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Ammonium sulfate precipitation
Formulation
PBS, 0.5% proprietary protein stabilizer
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: eIF2A
Alternate Names
EIF-2, EIF-2A, EIF2AEIF2, EIF-2alpha, eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa, subunit 1 (alpha, 35kD )
Entrez Gene IDs
83939 (Human)
Gene Symbol
EIF2A
UniProt
Additional eIF2A Products
Product Documents for eIF2A Antibody (3A7B11) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for eIF2A Antibody (3A7B11) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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