eIF2A Antibody (8V8H7)

Novus Biologicals | Catalog # NBP3-16864

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8V8H7 expressed in HEK293
View all eIF2A Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 450-550 of human eIF2A (Q9BY44). ALRNKPITNSKLHEEEPPQNMKPQSGNDKPLSKTALKNQRKHEAKKAAKQEARSDKSPDLAPTPAPQSTPRNTVSQSISGDPEIDKKIKNLKKKLKAIEQL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for eIF2A Antibody (8V8H7)

Western Blot: eIF2A Antibody (8V8H7) [NBP3-16864]

Western Blot: eIF2A Antibody (8V8H7) [NBP3-16864]

Western Blot: eIF2A Antibody (8V8H7) [NBP3-16864] - Western blot analysis of extracts of various cell lines, using eIF2A Rabbit mAb (NBP3-16864) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry-Paraffin: eIF2A Antibody (8V8H7) [NBP3-16864]

Immunohistochemistry-Paraffin: eIF2A Antibody (8V8H7) [NBP3-16864]

Immunohistochemistry-Paraffin: eIF2A Antibody (8V8H7) [NBP3-16864] - Immunohistochemistry of paraffin-embedded mouse testis using eIF2A Rabbit mAb (NBP3-16864) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western Blot: eIF2A Antibody (8V8H7) [NBP3-16864]

Western Blot: eIF2A Antibody (8V8H7) [NBP3-16864]

Western Blot: eIF2A Antibody (8V8H7) [NBP3-16864] - Western blot analysis of extracts of Rat lung, using eIF2A Rabbit mAb (NBP3-16864) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: eIF2A Antibody (8V8H7) [NBP3-16864]

Immunohistochemistry-Paraffin: eIF2A Antibody (8V8H7) [NBP3-16864]

Immunohistochemistry-Paraffin: eIF2A Antibody (8V8H7) [NBP3-16864] - Immunohistochemistry of paraffin-embedded rat pancreas using eIF2A Rabbit mAb (NBP3-16864) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
eIF2A Antibody (8V8H7)

Immunohistochemistry-Paraffin: eIF2A Antibody (8V8H7) [NBP3-16864] -

Immunohistochemistry-Paraffin: eIF2A Antibody (8V8H7) [NBP3-16864] -Analysis of paraffin-embedded Human tonsil tissue using eIF2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Applications for eIF2A Antibody (8V8H7)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunohistochemistry

1:200 - 1:2000

Immunohistochemistry-Paraffin

1:200 - 1:2000

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: eIF2A

The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM]

Alternate Names

EIF-2, EIF-2A, EIF2AEIF2, EIF-2alpha, eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa, subunit 1 (alpha, 35kD )

Gene Symbol

EIF2A

Additional eIF2A Products

Product Documents for eIF2A Antibody (8V8H7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for eIF2A Antibody (8V8H7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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