EIF3D Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-38217

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EIF3D Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human EIF3D (NP_003744.1).

Sequence:
MAKFMTPVIQDNPSGWGPCAVPEQFRDMPYQPFSKGDRLGKVADWTGATYQDKRYTNKYSSQFGGGSQYAYFHEEDESSFQLVDTARTQKTAYQRNRMRFAQRNLRRDKDRRNMLQFNLQILPKSAKQKERERIRLQKKFQKQFGVRQKWDQKSQKPRDSSVEVRSDWEVKEEMDFPQLMKMRYLEVSEPQDIECCGALEYYDKAFDRITTRSEKPLRSIKRIFHTVTTTDDPVIRKLAK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EIF3D Antibody - BSA Free

EIF3D Antibody

Immunocytochemistry/ Immunofluorescence: EIF3D Antibody [NBP3-38217] -

Immunocytochemistry/ Immunofluorescence: EIF3D Antibody [NBP3-38217] - Immunofluorescence analysis of L-929 cells using EIF3D Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
EIF3D Antibody

Immunocytochemistry/ Immunofluorescence: EIF3D Antibody [NBP3-38217] -

Immunocytochemistry/ Immunofluorescence: EIF3D Antibody [NBP3-38217] - Immunofluorescence analysis of C6 cells using EIF3D Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
EIF3D Antibody

Immunocytochemistry/ Immunofluorescence: EIF3D Antibody [NBP3-38217] -

Immunocytochemistry/ Immunofluorescence: EIF3D Antibody [NBP3-38217] - Immunofluorescence analysis of U-2 OS cells using EIF3D Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
EIF3D Antibody

Immunocytochemistry/ Immunofluorescence: EIF3D Antibody [NBP3-38217] -

Immunocytochemistry/ Immunofluorescence: EIF3D Antibody [NBP3-38217] - Immunofluorescence analysis of L-929 cells using EIF3D Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
EIF3D Antibody

Western Blot: EIF3D Antibody [NBP3-38217] -

Western Blot: EIF3D Antibody [NBP3-38217] - Western blot analysis of various lysates using EIF3D Rabbit pAb at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Applications for EIF3D Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:200 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EIF3D

Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex. [provided by RefSeq]

Alternate Names

eIF3d, eIF3-p66, EIF3S7eIF3 p66, eIF-3-zeta, eIF3-zeta, Eukaryotic translation initiation factor 3 subunit 7, eukaryotic translation initiation factor 3 subunit D, eukaryotic translation initiation factor 3, subunit 7 zeta, 66/67kDa, eukaryotic translation initiation factor 3, subunit D, MGC126526, MGC17258, translation initiation factor eIF3 p66 subunit

Gene Symbol

EIF3D

Additional EIF3D Products

Product Documents for EIF3D Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for EIF3D Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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