eIF4E Antibody (PCRP-EIF4E-1D3)

Novus Biologicals | Catalog # NBP3-07141

Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # PCRP-EIF4E-1D3
View all eIF4E Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human eIF4E protein (Uniprot: P06730)

Localization

Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

25.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08884)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for eIF4E Antibody (PCRP-EIF4E-1D3)

Immunocytochemistry/ Immunofluorescence: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141]

Immunocytochemistry/ Immunofluorescence: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141]

Immunocytochemistry/Immunofluorescence: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells using eIF4E Mouse Monoclonal Antibody (PCRP-EIF4E-1D3) followed by goat anti- Mouse IgG-CF488 (green), phalloidin (red).
Flow Cytometry: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141]

Flow Cytometry: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141]

Flow Cytometry: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141] - Flow cytometric analysis of PFA-fixed U87 cells. eIF4E antibody (PCRP-EIF4E-1D3) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).
Immunocytochemistry/ Immunofluorescence: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141]

Immunocytochemistry/ Immunofluorescence: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141]

Immunocytochemistry/Immunofluorescence: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141] - Immunofluorescence Analysis of PFA-fixed HeLa cells using eIF4E Mouse Monoclonal Antibody (PCRP-EIF4E-1D3) followed by goat anti- Mouse IgG-CF488 (green).
Flow Cytometry: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141]

Flow Cytometry: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141]

Flow Cytometry: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141] - Flow cytometric analysis of PFA-fixed HeLa cells. eIF4E Mouse Monoclonal Antibody (PCRP-eIF4E-1D3) followed by goat anti- Mouse IgG-CF488 (blue), isotype control (red).
Flow Cytometry: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141]

Flow Cytometry: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141]

Flow Cytometry: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141] - Flow cytometric analysis of PFA-fixed Raji cells.eIF4E Mouse Monoclonal Antibody (PCRP-eIF4E-1D3) followed by goat anti- Mouse IgG-CF488 (blue), isotype control (red).
Protein Array: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141]

Protein Array: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141]

Protein Array: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141] - Analysis of Protein Array containing more than 19,000 full-length human proteins using eIF4E Mouse Monoclonal Antibody (PCRP-eIF4E-1D3).

Applications for eIF4E Antibody (PCRP-EIF4E-1D3)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: eIF4E

eIF-4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It exists in two forms: as a free form (25 kDa) and as part of a multiprotein complex eIF-4F (1). eIF-4E appears to be the least abundant of the initiation factors and acts as a rate-limiting step of initiation (2). Since translation is regulated by phosphorylation, eIF-4E phosphorylation at Ser 209 by MAPK signal-integrating kinase 1 (Mnk1) and kinase 2 (Mnk2) may directly regulate the rate of protein synthesis initiation (3). There is also evidence that eIF-4E can function as an oncogene (4-5).

Long Name

Eukaryotic Translation Initiation Factor 4E

Alternate Names

EIF4EL1, EIF4F

Gene Symbol

EIF4E

UniProt

P06730

Additional eIF4E Products

Product Documents for eIF4E Antibody (PCRP-EIF4E-1D3)

Certificate of Analysis

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Product Specific Notices for eIF4E Antibody (PCRP-EIF4E-1D3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Interferons
Translation Regulation

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Protocols

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