eIF5A Antibody (4D1) - BSA Free
Novus Biologicals | Catalog # NBP2-59403
![Western Blot: eIF5A Antibody (4D1)BSA Free [NBP2-59403]](https://resources.rndsystems.com/images/products/eIF5A-Antibody-4D1-Western-Blot-NBP2-59403-img0001.jpg "Western Blot: eIF5A Antibody (4D1)BSA Free [NBP2-59403]")
Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 4D1
Format
BSA Free
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Product Specifications
Immunogen
Recombinant human eIF5A (1-154aa) purified from E. coli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for eIF5A Antibody (4D1) - BSA Free
Western Blot: eIF5A Antibody (4D1)BSA Free [NBP2-59403]
Western Blot: eIF5A Antibody (4D1) [NBP2-59403] - Lane 1: Jurkat cell lysates, Lane 2: 293T cell lysates, Lane 3: HeLa cell lysates, Lane 4: MCF7 cell lysates, Lane 5: NIH-3T3 cell lysates![Immunocytochemistry/ Immunofluorescence: eIF5A Antibody (4D1) - BSA Free [NBP2-59403]](https://resources.rndsystems.com/images/products/eIF5A-Antibody-4D1-Immunocytochemistry-Immunofluorescence-NBP2-59403-img0002.jpg "Immunocytochemistry/ Immunofluorescence: eIF5A Antibody (4D1) - BSA Free [NBP2-59403]")
Immunocytochemistry/ Immunofluorescence: eIF5A Antibody (4D1) - BSA Free [NBP2-59403]
Immunocytochemistry/Immunofluorescence: eIF5A Antibody (4D1) [NBP2-59403] - Analysis of EIF5A in Balb/3T3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human EIF5A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)![Flow Cytometry: eIF5A Antibody (4D1) - BSA Free [NBP2-59403]](https://resources.rndsystems.com/images/products/eIF5A-Antibody-4D1-Flow-Cytometry-NBP2-59403-img0003.jpg "Flow Cytometry: eIF5A Antibody (4D1) - BSA Free [NBP2-59403]")
Flow Cytometry: eIF5A Antibody (4D1) - BSA Free [NBP2-59403]
Flow Cytometry: eIF5A Antibody (4D1) [NBP2-59403] - Analysis of EIF5A in Balb/3T3 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line)Applications for eIF5A Antibody (4D1) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1015
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:500
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: eIF5A
Long Name
Eukaryotic Translation Initiation Factor 5A
Alternate Names
eIF4D, Rev-binding Factor
Gene Symbol
EIF5A
Additional eIF5A Products
Product Documents for eIF5A Antibody (4D1) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for eIF5A Antibody (4D1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for eIF5A Antibody (4D1) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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