eIF5A Antibody (8C1) - Azide and BSA Free

Novus Biologicals | Catalog # H00001984-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 8C1

Format

Azide and BSA Free
View all eIF5A Primary Antibodies »

Product Specifications

Immunogen

EIF5A (NP_001961.1, 1 a.a. ~ 154 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MADDLDFETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQDGYLSLLQDSGEVREDLRLPEGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIKAMAK

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for eIF5A Antibody (8C1) - Azide and BSA Free

Western Blot: eIF5A Antibody (8C1) [H00001984-M01]

Western Blot: eIF5A Antibody (8C1) [H00001984-M01]

Western Blot: eIF5A Antibody (8C1) [H00001984-M01] - Analysis of EIF5A expression in transfected 293T cell line by EIF5A monoclonal antibody (M01), clone 8C1.Lane 1: EIF5A transfected lysate(16.8 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: eIF5A Antibody (8C1) [H00001984-M01]

Immunocytochemistry/ Immunofluorescence: eIF5A Antibody (8C1) [H00001984-M01]

Immunocytochemistry/Immunofluorescence: eIF5A Antibody (8C1) [H00001984-M01] - Analysis of monoclonal antibody to EIF5A on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: eIF5A Antibody (8C1) [H00001984-M01] - Detection limit for recombinant GST tagged EIF5A is 3 ng/ml as a capture antibody.

Applications for eIF5A Antibody (8C1) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA and WB.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: eIF5A

The human eukaryotic translation initiation factor 5A (eIF5A) is an 18 kDa protein. eIF5A precursor [(ec-eIF-5A(lys)] is the only cellular protein known to contain a specific lysine residue which is transformed into the unique amino acid hypusine [N-(4-amino-2-hydroxybutyl)-lysine] by a series of post translational reactions. eIF5A promotes the formation of the first peptide bond during the initial stage of protein synthesis.

Long Name

Eukaryotic Translation Initiation Factor 5A

Alternate Names

eIF4D, Rev-binding Factor

Gene Symbol

EIF5A

UniProt

NP_001961 (Human)

Additional eIF5A Products

Product Documents for eIF5A Antibody (8C1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for eIF5A Antibody (8C1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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