ELISpot Blue Color Module (Strep-AP and BCIP-NBT), 5 Plate

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R&D Systems ELISpot Development Modules
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Citations (34)
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ELISpot Blue Color Module (Strep-AP and BCIP-NBT), 5 Plate Summary

Assay Type
Quantitative Sandwich ELISA Development Module
96-well microplate, sold separately (see Other Reagents Required)
Assay Length
3 hours 35 mins to 4 hours 50 mins**
Sample Type
Whole Cells
Sufficient Materials
For five 96-well microplates
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Complete ELISpot kits are highly sensitive, microplate-based assays for the detection of cytokine secreting cells. Kits are available for detection and enumeration of a single analyte or two analytes simultaneously. Complete ELISpot kits are ready-to-run and require no assay development or refinement. ELISpot Development Modules contain the basic components required to develop an ELISpot assay. They offer an economical alternative to buying separate antibodies.

ELISpot development modules are an alternative to ELISpot kits. A basic understanding of ELISpot assay development is required for the successful use of these reagents. Each investigator should optimize the coating conditions, the assay sensitivity, the type of enzyme and substrate, as well as the concentrations of the capture and detection antibodies to achieve desired results. The analyte-specific ELISpot Development Module and the ELISpot Blue Color Module contain the necessary components for analyte detection and visualization, respectively. These modules can be used together but are sold separately. Each module contains enough reagents for at least five 96-well microplates.


  • An economical alternative to ELISpot Kits
  • Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time
  • Generic development protocols provide direction to start an optimization protocol
  • Customize the assay to your specific needs



  • Streptavidin-AP Concentrate
  • BCIP/NBT Chromogen



  • PBS - 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
  • Wash Buffer - 0.05% Tween® 20 in PBS
  • Reagent Diluent - 1% BSA in PBS, pH 7.2 - 7.4, 0.2 µm filtered
  • Deionized H2O


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Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
⚠ WARNING: This product can expose you to chemicals including methanol, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov.

Citations for ELISpot Blue Color Module (Strep-AP and BCIP-NBT), 5 Plate

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

34 Citations: Showing 1 - 10
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  1. Evaluation of the Delivery of a Live Attenuated Porcine Reproductive and Respiratory Syndrome Virus as a Unit Solid Dose Injectable Vaccine
    Authors: E Hayhurst, E Rose, M Pedrera, JC Edwards, N Kotynska, D Grainger, Y Sadigh, J Flannery, L Bonnet, R Ritwik, P Dulal, MK Howard, SP Graham
    Vaccines, 2022;10(11):.
    Species: Porcine
    Sample Types: Whole Cells
  2. Early human B cell signatures of the primary antibody response to mRNA vaccination
    Authors: L Kardava, N Rachmanino, WW Lau, CM Buckner, K Trihemasav, J Blazkova, F Lopes de A, W Wang, X Zhang, Y Wang, CI Chiang, S Narpala, GE McCormack, C Liu, CA Seamon, MC Sneller, S O'Connell, Y Li, AB McDermott, TW Chun, AS Fauci, JS Tsang, S Moir
    Oncogene, 2022;119(28):e2204607119.
    Species: Human
    Sample Types: Whole Cells
  3. Pre-vaccination and early B cell signatures predict antibody response to SARS-CoV-2 mRNA vaccine
    Authors: L Kardava, N Rachmanino, WW Lau, CM Buckner, K Trihemasav, FL de Assis, W Wang, X Zhang, Y Wang, CI Chiang, S Narpala, R Reger, GE McCormack, CA Seamon, RW Childs, AF Suffredini, JR Strich, DS Chertow, RT Davey, MC Sneller, S Oâ Connell, Y Li, A McDermott, TW Chun, AS Fauci, JS Tsang, S Moir
    medRxiv : the preprint server for health sciences, 2021;0(0):.
    Species: Human
    Sample Types: Whole Cells
  4. Synthetic DNA Delivery of an Engineered Arginase Enzyme Can Modulate Specific Immunity In�Vivo
    Authors: M Khoshnejad, A Perales-Pu, Y Dia, P Xiao, A Patel, Z Xu, X Zhu, K Yun, I Baboo, R Qureshi, L Humeau, K Muthumani, DB Weiner
    Mol Ther Methods Clin Dev, 2020;18(0):652-663.
    Species: Mouse
    Sample Types: Whole Cells
  5. Modifying bacterial flagellin to evade Nod-like Receptor CARD 4 recognition enhances protective immunity against Salmonella
    Authors: P Tourlomous, JA Wright, AS Bittante, LJ Hopkins, SJ Webster, OJ Bryant, P Mastroeni, DJ Maskell, CE Bryant
    Nat Microbiol, 2020;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  6. Protective immunity by an engineered DNA vaccine for Mayaro virus
    Authors: H Choi, SB Kudchodkar, EL Reuschel, K Asija, P Borole, M Ho, K Wojtak, C Reed, S Ramos, NE Bopp, PV Aguilar, SC Weaver, JJ Kim, L Humeau, P Tebas, DB Weiner, K Muthumani
    PLoS Negl Trop Dis, 2019;13(2):e0007042.
    Species: Mouse
    Sample Types: Whole Cells
  7. Enhancement of Immune Responses by Guanosine-Based Particles in DNA Plasmid Formulations against Infectious Diseases
    Authors: S Santos, M Ramírez, E Miranda, N Reyes, O Martínez, M Acosta-San, JM Rivera, M Otero
    J Immunol Res, 2019;2019(0):3409371.
    Species: Mouse
    Sample Types: Whole Cells
  8. Restoration of T Cell function in multi-drug resistant bacterial sepsis after interleukin-7, anti-PD-L1, and OX-40 administration
    Authors: LK Thampy, KE Remy, AH Walton, Z Hong, K Liu, R Liu, V Yi, CD Burnham, RS Hotchkiss
    PLoS ONE, 2018;13(6):e0199497.
    Species: Human
    Sample Types: Whole Cells
  9. Characterization of the immune response elicited by the vaccinia virus L3 protein delivered as naked DNA
    Authors: M Ramírez, S Santos, O Martínez, R Rodríguez, E Miranda, WD Ramos-Pere, M Otero
    Vaccine, 2018;36(15):2049-2055.
    Species: Mouse
    Sample Types: Whole Cells
  10. Donor bone marrow cells are essential for iNKT cell-mediated Foxp3+ Treg cell expansion in a murine model of transplantation tolerance
    Authors: S Miyairi, T Hirai, R Ishii, M Okumi, S Nunoda, K Yamazaki, Y Ishii, K Tanabe
    Eur. J. Immunol, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
  11. Effective Depletion of Pre-existing Anti-AAV Antibodies Requires Broad Immune Targeting
    Authors: VM Velazquez, AS Meadows, RJ Pineda, M Camboni, DM McCarty, H Fu
    Mol Ther Methods Clin Dev, 2017;4(0):159-168.
    Species: Mouse
    Sample Types: Whole Cells
  12. Cerium Oxide Nanoparticles in Lung Acutely Induce Oxidative Stress, Inflammation, and DNA Damage in Various Organs of Mice
    Authors: A Nemmar, P Yuvaraju, S Beegam, MA Fahim, BH Ali
    Oxid Med Cell Longev, 2017;2017(0):9639035.
    Species: Mouse
    Sample Types: Tissue Homogenates
  13. Rad52 deficiency decreases development of lung squamous cell carcinomas by enhancing immuno-surveillance
    Authors: R Lieberman, J Pan, Q Zhang, M You
    Oncotarget, 2017;8(21):34032-34044.
    Species: Mouse
    Sample Types: Whole Cells
  14. Characterization of guinea pig T cell responses elicited after EP-assisted delivery of DNA vaccines to the skin
    Authors: Trevor R F Smith
    Vaccine, 2017;35(1):61-70.
  15. Preclinical evaluation of multi antigenic HCV DNA vaccine for the prevention of Hepatitis C virus infection
    Authors: H Lee, M Jeong, J Oh, Y Cho, X Shen, J Stone, J Yan, Z Rothkopf, AS Khan, BM Cho, YK Park, DB Weiner, WC Son, JN Maslow
    Sci Rep, 2017;7(0):43531.
    Species: Mouse
    Sample Types: Whole Cells
  16. CD4(+) and CD8(+) TCR? repertoires possess different potentials to generate extraordinarily high-avidity T cells
    Authors: M Nakatsugaw, MA Rahman, Y Yamashita, T Ochi, P Wnuk, S Tanaka, K Chamoto, Y Kagoya, K Saso, T Guo, M Anczurowsk, MO Butler, N Hirano
    Sci Rep, 2016;6(0):23821.
    Species: Human
    Sample Types: Whole Cells
  17. Autoreactive CD19+CD20- Plasma Cells Contribute to Disease Severity of Experimental Autoimmune Encephalomyelitis.
    Authors: Chen D, Ireland S, Davis L, Kong X, Stowe A, Wang Y, White W, Herbst R, Monson N
    J Immunol, 2016;196(4):1541-9.
    Species: Mouse
    Sample Types: Whole Cells
  18. HIV-1 Env DNA vaccine plus protein boost delivered by EP expands B- and T-cell responses and neutralizing phenotype in vivo.
    Authors: Muthumani, Kar, Wise, Megan C, Broderick, Kate E, Hutnick, Natalie, Goodman, Jonathan, Flingai, Seleeke, Yan, Jian, Bian, Chaoran, Mendoza, Janess, Tingey, Colleen, Wilson, Christin, Wojtak, Krzyszto, Sardesai, Niranjan, Weiner, David B
    PLoS ONE, 2013;8(12):e84234.
    Species: Mouse
    Sample Types: Whole Cells
  19. Proteome-wide screening reveals immunodominance in the CD8 T cell response against classical swine fever virus with antigen-specificity dependent on MHC class I haplotype expression.
    Authors: Franzoni G, Kurkure N, Essler S, Pedrera M, Everett H, Bodman-Smith K, Crooke H, Graham S
    PLoS ONE, 2013;8(12):e84246.
    Species: Porcine
    Sample Types: Whole Cells
  20. Nanoparticle vaccines encompassing the respiratory syncytial virus (RSV) G protein CX3C chemokine motif induce robust immunity protecting from challenge and disease.
    Authors: Jorquera P, Choi Y, Oakley K, Powell T, Boyd J, Palath N, Haynes L, Anderson L, Tripp R
    PLoS ONE, 2013;8(9):e74905.
    Species: Mouse
    Sample Types: Whole Cells
  21. Systemic immunization with CCL27/CTACK modulates immune responses at mucosal sites in mice and macaques.
    Authors: Kraynyak KA, Kutzler MA, Cisper NJ, Khan AS, Draghia-Akli R, Sardesal NY, Lewis MG, Yan J, Weiner DB
    Vaccine, 2010;28(8):1942-51.
  22. Immune responses to AAV in canine muscle monitored by cellular assays and noninvasive imaging.
    Authors: Wang Z, Storb R, Lee D, Kushmerick MJ, Chu B, Berger C, Arnett A, Allen J, Chamberlain JS, Riddell SR, Tapscott SJ
    Mol. Ther., 2010;18(3):617-24.
    Species: Canine
    Sample Types: Whole Cells
  23. Impact of the underlying mutation and the route of vector administration on immune responses to factor IX in gene therapy for hemophilia B.
    Authors: Cao O, Hoffman BE, Moghimi B, Nayak S, Cooper M, Zhou S, Ertl HC, High KA, Herzog RW
    Mol. Ther., 2009;17(10):1733-42.
    Species: Mouse
    Sample Types: Whole Cells
  24. Antiglioma immunological memory in response to conditional cytotoxic/immune-stimulatory gene therapy: humoral and cellular immunity lead to tumor regression.
    Authors: Ghulam Muhammad AK, Candolfi M, King GD, Yagiz K, Foulad D, Mineharu Y, Kroeger KM, Treuer KA, Nichols WS, Sanderson NS, Yang J, Khayznikov M, Van Rooijen N, Lowenstein PR, Castro MG
    Clin. Cancer Res., 2009;15(19):6113-27.
    Species: Rat
    Sample Types: Whole Cells
  25. Plasmid-encoded interleukin-15 receptor alpha enhances specific immune responses induced by a DNA vaccine in vivo.
    Authors: Kraynyak KA, Kutzler MA, Cisper NJ, Laddy DJ, Morrow MP, Waldmann TA, Weiner DB
    Hum. Gene Ther., 2009;20(10):1143-56.
    Species: Mouse
    Sample Types:
  26. Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
    Authors: Alisa A, Boswell S, Pathan AA, Ayaru L, Williams R, Behboudi S
    J. Immunol., 2008;180(7):5109-17.
    Species: Human
    Sample Types: Whole Cells
  27. Evidence for HIV-associated B cell exhaustion in a dysfunctional memory B cell compartment in HIV-infected viremic individuals.
    Authors: Moir S, Ho J, Malaspina A, Wang W, DiPoto AC, O'Shea MA, Roby G, Kottilil S, Arthos J, Proschan MA, Chun TW, Fauci AS
    J. Exp. Med., 2008;205(8):1797-805.
    Species: N/A
    Sample Types: N/A
  28. Immunogenicity of novel consensus-based DNA vaccines against Chikungunya virus.
    Authors: Muthumani K, Lankaraman KM, Laddy DJ, Sundaram SG, Chung CW, Sako E, Wu L, Khan A, Sardesai N, Kim JJ, Vijayachari P, Weiner DB
    Vaccine, 2008;26(40):5128-34.
    Species: Mouse
    Sample Types: Whole Cells
  29. DR5 activation of caspase-8 induces DC maturation and immune enhancement in vivo.
    Authors: Chattergoon MA, Muthumani K, Tamura Y, Ramanathan M, Shames JP, Saulino V, Robinson TM, Montaner LJ, Weiner DB
    Mol. Ther., 2007;16(2):419-26.
    Species: Mouse
    Sample Types: Whole Cells
  30. Vaccination with recombinant fusion proteins incorporating Toll-like receptor ligands induces rapid cellular and humoral immunity.
    Authors: Huleatt JW, Jacobs AR, Tang J, Desai P, Kopp EB, Huang Y, Song L, Nakaar V, Powell TJ
    Vaccine, 2007;25(4):763-75.
    Species: Mouse
    Sample Types: Whole Cells
  31. Mapping of immune responses following wild-type and mutant ABeta42 plasmid or peptide vaccination in different mouse haplotypes and HLA Class II transgenic mice.
    Authors: Kutzler MA, Cao C, Bai Y, Dong H, Choe PY, Saulino V, McLaughlin L, Whelan A, Choo AY, Weiner DB, Ugen KE
    Vaccine, 2006;24(21):4630-9.
    Species: Mouse
    Sample Types: Whole Cells
  32. Developing an epitope-driven tuberculosis (TB) vaccine.
    Authors: De Groot AS, McMurry J, Marcon L, Franco J, Rivera D, Kutzler M, Weiner D, Martin B
    Vaccine, 2005;23(17):2121-31.
    Species: Mouse
    Sample Types:
  33. Therapeutic effectiveness of recombinant cancer vaccines is associated with a prevalent T-cell receptor alpha usage by melanoma-specific CD8+ T lymphocytes.
    Authors: De Palma R, Marigo I, Del Galdo F, De Santo C, Serafini P, Cingarlini S, Tuting T, Lenz J, Basso G, Milan G, Zanovello P, Bronte V
    Cancer Res., 2004;64(21):8068-76.
    Species: Mouse
    Sample Types:
  34. Co-immunization with plasmid IL-12 generates a strong T-cell memory response in mice.
    Authors: Chattergoon MA, Saulino V, Shames JP, Stein J, Montaner LJ, Weiner DB
    Vaccine, 2004;22(13):1744-50.
    Species: Mouse
    Sample Types: Whole Cells


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