ELK3 Antibody (OTI1H3)

Novus Biologicals | Catalog # NBP2-01264

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1H3
View all ELK3 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ELK3(NP_005221) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

44.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ELK3 Antibody (OTI1H3)

Western Blot: ELK3 Antibody (OTI1H3) [NBP2-01264]

Western Blot: ELK3 Antibody (OTI1H3) [NBP2-01264]

Western Blot: ELK3 Antibody (OTI1H3) [NBP2-01264] - Breast cancer cells. Lane 1: MDA-MB231 + Negative control siRNA. Lane 2: MDA-MB231 + siELK3-1. Lane 3: MDA-MB231 + siELK3-2. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: ELK3 Antibody (1H3) [NBP2-01264] Staining of COS7 cells transiently transfected by pCMV6-ENTRY ELK3.
Immunohistochemistry-Paraffin: ELK3 Antibody (OTI1H3) [NBP2-01264]

Immunohistochemistry-Paraffin: ELK3 Antibody (OTI1H3) [NBP2-01264]

Immunohistochemistry-Paraffin: ELK3 Antibody (1H3) [NBP2-01264] - Staining of paraffin-embedded Human tonsil using anti-ELK3 mouse monoclonal antibody.
Flow Cytometry: ELK3 Antibody (OTI1H3) [NBP2-01264]

Flow Cytometry: ELK3 Antibody (OTI1H3) [NBP2-01264]

Flow Cytometry: ELK3 Antibody (1H3) [NBP2-01264] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ELK3 antibody, and then analyzed by flow cytometry.
Western Blot: ELK3 Antibody (OTI1H3) [NBP2-01264]

Western Blot: ELK3 Antibody (OTI1H3) [NBP2-01264]

Western Blot: ELK3 Antibody (1H3) [NBP2-01264] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ELK3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ELK3.
Immunohistochemistry-Paraffin: ELK3 Antibody (OTI1H3) [NBP2-01264]

Immunohistochemistry-Paraffin: ELK3 Antibody (OTI1H3) [NBP2-01264]

Immunohistochemistry-Paraffin: ELK3 Antibody (1H3) [NBP2-01264] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ELK3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ELK3 Antibody (OTI1H3) [NBP2-01264]

Immunohistochemistry-Paraffin: ELK3 Antibody (OTI1H3) [NBP2-01264]

Immunohistochemistry-Paraffin: ELK3 Antibody (1H3) [NBP2-01264] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ELK3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ELK3 Antibody (OTI1H3) [NBP2-01264]

Immunohistochemistry-Paraffin: ELK3 Antibody (OTI1H3) [NBP2-01264]

Immunohistochemistry-Paraffin: ELK3 Antibody (1H3) [NBP2-01264] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ELK3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ELK3 Antibody (OTI1H3) [NBP2-01264]

Immunohistochemistry-Paraffin: ELK3 Antibody (OTI1H3) [NBP2-01264]

Immunohistochemistry-Paraffin: ELK3 Antibody (1H3) [NBP2-01264] - Staining of paraffin-embedded Human lymphoma tissue using anti-ELK3 mouse monoclonal antibody.
ELK3 Antibody (OTI1H3)

Western Blot: ELK3 Antibody (OTI1H3) [NBP2-01264] -

ELK3 increases the migration and invasion of SNU484 cell line in E-cadherin independent manner. (A) ELK3 mRNA levels in SNU484 cells transfected with control vector and pLenti-cMyc-DDK-ELK3 plasmid. Error bars represent the standard deviation; **** p < 0.0001 (student’s t-test). (B) ELK3 protein levels in SNU484 cells transfected with control vector and pLenti-cMyc-DDK-ELK3 plasmid. The values indicate the signal intensities of ELK3 relative to GAPDH. (C) Representative images showing cell migration (top two panels) and cell invasion (bottom two panels) of SNU484 cells transfected with control vector and pLenti-cMyc-DDK-ELK3 plasmid. Scale bar, 200 μm. Bar graphs indicate the number of migrated cells and invaded cells. Error bars represent the standard deviation; ** p < 0.01 and *** p < 0.001 (student’s t-test). (D) Epithelial marker (E-cadherin), mesenchymal marker (Fibronectin), and ELK3 protein expression in SNU484 cells transfected with control vector and ELK3. Bar graphs indicate the quantified levels of each protein, in relative scales. Error bars represent the standard deviation; * p < 0.05 and *** p < 0.001 (student’s t-test). ELK3, ETS transcription factor ELK3; Vector, control vector. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35409069), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
ELK3 Antibody (OTI1H3)

Western Blot: ELK3 Antibody (OTI1H3) [NBP2-01264] -

Depletion of ELK3 decreases the migration and invasion of SNU638 cell line in E-cadherin independent manner. (A) ELK3 mRNA levels in SNU638 cells transfected with siCon or siELK3. Error bars represent the standard deviation; **** p < 0.0001 (student’s t-test). (B) ELK3 protein levels in SNU638 cells transfected with siCon or siELK3. The values indicate the signal intensities of ELK3 relative to GAPDH. (C) Representative images showing cell migration (top two panels) and cell invasion (bottom two panels) of SNU638 cells transfected with siCon or siELK3. Scale bar, 200 μm. Bar graphs indicate the number of migrated and invaded cells, respectively. Error bars represent the standard deviation; * p < 0.05 and ** p < 0.01 (student’s t-test). (D) Epithelial marker (E-cadherin), mesenchymal marker (Fibronectin), and ELK3 protein expression in SNU638 cells transfected with siCon or siELK3. Bar graphs indicate the quantified levels of each protein, in relative scales. Error bars represent the standard deviation; ** p < 0.01 (student’s t-test). ELK3, ETS transcription factor ELK3; si, small interfering RNA; Con, control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35409069), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
ELK3 Antibody (OTI1H3)

Western Blot: ELK3 Antibody (OTI1H3) [NBP2-01264] -

ELK3 increases the migration and invasion of SNU484 cell line in E-cadherin independent manner. (A) ELK3 mRNA levels in SNU484 cells transfected with control vector and pLenti-cMyc-DDK-ELK3 plasmid. Error bars represent the standard deviation; **** p < 0.0001 (student’s t-test). (B) ELK3 protein levels in SNU484 cells transfected with control vector and pLenti-cMyc-DDK-ELK3 plasmid. The values indicate the signal intensities of ELK3 relative to GAPDH. (C) Representative images showing cell migration (top two panels) and cell invasion (bottom two panels) of SNU484 cells transfected with control vector and pLenti-cMyc-DDK-ELK3 plasmid. Scale bar, 200 μm. Bar graphs indicate the number of migrated cells and invaded cells. Error bars represent the standard deviation; ** p < 0.01 and *** p < 0.001 (student’s t-test). (D) Epithelial marker (E-cadherin), mesenchymal marker (Fibronectin), and ELK3 protein expression in SNU484 cells transfected with control vector and ELK3. Bar graphs indicate the quantified levels of each protein, in relative scales. Error bars represent the standard deviation; * p < 0.05 and *** p < 0.001 (student’s t-test). ELK3, ETS transcription factor ELK3; Vector, control vector. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35409069), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
ELK3 Antibody (OTI1H3)

Western Blot: ELK3 Antibody (OTI1H3) [NBP2-01264] -

ETS transcription factor ELK3 (ELK3) expression pattern in various gastric cancer cell lines. (A) ELK3 expression in gastric cancer cell lines from the Cancer Dependency Map (DepMap) database. (B) ELK3 mRNA expression in five gastric cancer cell lines, measured by reverse transcription-quantitative PCR. Error bars represent the standard deviation; ** p < 0.01 (one-way ANOVA). (C) ELK3 protein levels in five gastric cancer cell lines. The values indicate the signal intensities of ELK3 relative to GAPDH. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35409069), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
ELK3 Antibody (OTI1H3)

Western Blot: ELK3 Antibody (OTI1H3) [NBP2-01264] -

Depletion of ELK3 decreases the migration and invasion of SNU638 cell line in E-cadherin independent manner. (A) ELK3 mRNA levels in SNU638 cells transfected with siCon or siELK3. Error bars represent the standard deviation; **** p < 0.0001 (student’s t-test). (B) ELK3 protein levels in SNU638 cells transfected with siCon or siELK3. The values indicate the signal intensities of ELK3 relative to GAPDH. (C) Representative images showing cell migration (top two panels) and cell invasion (bottom two panels) of SNU638 cells transfected with siCon or siELK3. Scale bar, 200 μm. Bar graphs indicate the number of migrated and invaded cells, respectively. Error bars represent the standard deviation; * p < 0.05 and ** p < 0.01 (student’s t-test). (D) Epithelial marker (E-cadherin), mesenchymal marker (Fibronectin), and ELK3 protein expression in SNU638 cells transfected with siCon or siELK3. Bar graphs indicate the quantified levels of each protein, in relative scales. Error bars represent the standard deviation; ** p < 0.01 (student’s t-test). ELK3, ETS transcription factor ELK3; si, small interfering RNA; Con, control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35409069), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for ELK3 Antibody (OTI1H3)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Reviewed Applications

Read 1 review rated 5 using NBP2-01264 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ELK3

The protein encoded by this gene is a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. Proteins in this subfamily regulate transcription when recruited by serum response factor to bind to serum response elements. This protein is activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present. [provided by RefSeq].

Alternate Names

ELK3, ETS-domain protein (SRF accessory protein 2), ERPETS domain-containing protein Elk-3, ETS-domain protein, ETS-related protein ERP, NETSRF accessory protein 2, SAP-2, SAP2ETS-related protein NET, Serum response factor accessory protein 2

Entrez Gene IDs

2004 (Human)

Gene Symbol

ELK3

Additional ELK3 Products

Product Documents for ELK3 Antibody (OTI1H3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ELK3 Antibody (OTI1H3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ELK3 Antibody (OTI1H3)

Customer Reviews for ELK3 Antibody (OTI1H3) (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used ELK3 Antibody (OTI1H3)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • ELK3 Antibody (OTI1H3)
    Name: JIHOON PARK
    Application: Western Blot
    Sample Tested: Breast cancer cells
    Species: Human
    Verified Customer | Posted 07/17/2018
    1 : MDA-MB231 + Negative control siRNA 2 : MDA-MB231 + siELK3-1 3 : MDA-MB231 + siELK3-2
    ELK3 Antibody (OTI1H3) NBP2-01264

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies