Elongin A Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87040

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Elongin A Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ERHLGEPHGKGVVSQNKEHKSSHKDKRPVDAKSDEKASVVSREKSHKALSKEENRRPPSGDNAREKPPSSGVKKEKDREGSSLKKKCLPPSEAASDNHLKKPKHRDPEKAKLDKSKQGLDSFDTGKGAGD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Elongin A Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Elongin A Antibody [NBP1-87040]

Immunocytochemistry/ Immunofluorescence: Elongin A Antibody [NBP1-87040]

Immunocytochemistry/Immunofluorescence: Elongin A Antibody [NBP1-87040] - Staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Elongin A Antibody [NBP1-87040]

Immunohistochemistry-Paraffin: Elongin A Antibody [NBP1-87040]

Immunohistochemistry-Paraffin: Elongin A Antibody [NBP1-87040] - Staining of human liver shows moderate to strong nuclear positivity in hepatocytes.
Immunohistochemistry-Paraffin: Elongin A Antibody [NBP1-87040]

Immunohistochemistry-Paraffin: Elongin A Antibody [NBP1-87040]

Immunohistochemistry-Paraffin: Elongin A Antibody [NBP1-87040] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Elongin A Antibody [NBP1-87040]

Immunohistochemistry-Paraffin: Elongin A Antibody [NBP1-87040]

Immunohistochemistry-Paraffin: Elongin A Antibody [NBP1-87040] - Staining of human placenta shows moderate nuclear positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Elongin A Antibody [NBP1-87040]

Immunohistochemistry-Paraffin: Elongin A Antibody [NBP1-87040]

Immunohistochemistry-Paraffin: Elongin A Antibody [NBP1-87040] - Staining of human rectum shows moderate nuclear positivity in glandular cells.
Elongin A Antibody - BSA Free Western Blot: Elongin A Antibody - BSA Free [NBP1-87040]

Western Blot: Elongin A Antibody - BSA Free [NBP1-87040]

Analysis in human cell line U-2197.

Applications for Elongin A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Elongin A

TCEB3 (Elongin A) is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation.

Alternate Names

EloA, Elongin 110 kDa subunit, elongin-A, FLJ38760, FLJ42849, RNA polymerase II transcription factor SIII subunit A1, SIII, SIII p110, TCEB3A, transcription elongation factor B (SIII), polypeptide 3 (110kD, elongin A), transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A), transcription elongation factor B alpha subunit, transcription elongation factor B polypeptide 3

Gene Symbol

TCEB3

Additional Elongin A Products

Product Documents for Elongin A Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Elongin A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Elongin A Antibody - BSA Free

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Protocols

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FAQs

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