Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all EMAP-II Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human EMAP-II/AIMP1 (Accession#: NP_004748.2; Ala2-Lys312)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for EMAP-II/AIMP1 Antibody - Azide and BSA Free

Western Blot: EMAP-II/AIMP1 Antibody [NBP2-99128]

Western Blot: EMAP-II/AIMP1 Antibody [NBP2-99128]

Western Blot: EMAP-II/AIMP1 Antibody [NBP2-99128] - Anti-EMAP-II/AIMP1 rabbit polyclonal antibody at 1:500 dilution. Lane A: H1299 Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lane C: HEK293 Whole Cell Lysate. Lane D: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 38 kDa
Immunohistochemistry-Paraffin: EMAP-II/AIMP1 Antibody [NBP2-99128]

Immunohistochemistry-Paraffin: EMAP-II/AIMP1 Antibody [NBP2-99128]

Immunohistochemistry-Paraffin: EMAP-II/AIMP1 Antibody [NBP2-99128] - Staining of human EMAP-II/AIMP1 in human pancreas with rabbit polyclonal antibody at 1:5000 dilution.
Immunohistochemistry-Paraffin: EMAP-II/AIMP1 Antibody [NBP2-99128]

Immunohistochemistry-Paraffin: EMAP-II/AIMP1 Antibody [NBP2-99128]

Immunohistochemistry-Paraffin: EMAP-II/AIMP1 Antibody [NBP2-99128] - Staining of human EMAP-II/AIMP1 in human brain with rabbit polyclonal antibody at 1:5000 dilution.
Immunoprecipitation: EMAP-II/AIMP1 Antibody [NBP2-99128]

Immunoprecipitation: EMAP-II/AIMP1 Antibody [NBP2-99128]

Immunoprecipitation: EMAP-II/AIMP1 Antibody [NBP2-99128] - EMAP-II/AIMP1 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. 4 ul anti-EMAP-II/AIMP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-EMAP-II/AIMP1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 35 kDa
EMAP-II/AIMP1 Antibody - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: EMAP-II/AIMP1 Antibody [NBP2-99128] -

Immunocytochemistry/Immunofluorescence: EMAP-II/AIMP1 Antibody [NBP2-99128] - Immunofluorescence staining of EMAP-II/AIMP1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99128 antibody (dilution ratio 1:500) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Applications for EMAP-II/AIMP1 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry-Paraffin

1:1000-1:10000

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EMAP-II

EMAP II (Endothelial-Monocyte-Activating Polypeptide II) is a proinflammatory cytokine and chemoattractant of macrophages and polymorphonuclear lymphocytes. EMAP II is synthesized as a 34 kDa precursor molecule which is cleaved to a biologically active 22 kDa mature polypeptide. This active protein is known to induce the procoagulant protein tissue factor on the surface of endothelial cells and modulate other properties of endothelial cells and monocytes in vitro, including the stimulation of TNF-alpha and myeloperoxidase. EMAP II activates neutrophils in vivo and induces an inflammatory reaction and tumor regression. EMAP II induces apoptosis in proliferating endothelial cells and inhibits neovascularization. This anti-angiogenic property is of interest to tumor biology. EMAP II is also a negative modulator of lung vascular growth.

Long Name

Endothelial-Monocyte Activating Polypeptide II

Alternate Names

AIMP1, EMAPII, SCYE1

Gene Symbol

AIMP1

Additional EMAP-II Products

Product Documents for EMAP-II/AIMP1 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for EMAP-II/AIMP1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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