Emerin Antibody (CL0201) - BSA Free

Novus Biologicals | Catalog # NBP2-52876

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL0201

Format

BSA Free
View all Emerin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P50402, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Emerin Antibody (CL0201) - BSA Free

Western Blot: Emerin Antibody (CL0201) [NBP2-52876]

Western Blot: Emerin Antibody (CL0201) [NBP2-52876]

Western Blot: Emerin Antibody (CL0201) [NBP2-52876] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-EMD antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Western Blot: Emerin Antibody (CL0201) [NBP2-52876]

Western Blot: Emerin Antibody (CL0201) [NBP2-52876]

Western Blot: Emerin Antibody (CL0201) [NBP2-52876] - Lane 1: Marker [kDa] Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: Emerin Antibody (CL0201) [NBP2-52876]

Immunocytochemistry/ Immunofluorescence: Emerin Antibody (CL0201) [NBP2-52876]

Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0201) [NBP2-52876] - Staining of PC-3 cells using the Anti-EMD monoclonal antibody, showing specific staining in the nuclear membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Emerin Antibody (CL0201) [NBP2-52876]

Immunohistochemistry-Paraffin: Emerin Antibody (CL0201) [NBP2-52876]

Immunohistochemistry-Paraffin: Emerin Antibody (CL0201) [NBP2-52876] - Staining of human stomach shows strong positivity in the nuclear membrane of glandular cells.
Immunohistochemistry-Paraffin: Emerin Antibody (CL0201) [NBP2-52876]

Immunohistochemistry-Paraffin: Emerin Antibody (CL0201) [NBP2-52876]

Immunohistochemistry-Paraffin: Emerin Antibody (CL0201) [NBP2-52876] - Staining of human fallopian tube shows strong positivity in the nuclear membrane of glandular cells.
Immunohistochemistry-Paraffin: Emerin Antibody (CL0201) [NBP2-52876]

Immunohistochemistry-Paraffin: Emerin Antibody (CL0201) [NBP2-52876]

Immunohistochemistry-Paraffin: Emerin Antibody (CL0201) [NBP2-52876] - Staining of human kidney shows strong immunoreactivity in the nuclear membrane in renal glomeruli and tubuli.
Immunohistochemistry-Paraffin: Emerin Antibody (CL0201) [NBP2-52876]

Immunohistochemistry-Paraffin: Emerin Antibody (CL0201) [NBP2-52876]

Immunohistochemistry-Paraffin: Emerin Antibody (CL0201) [NBP2-52876] - Staining of human placenta shows strong immunoreactivity in the nuclear membrane in the trophoblast layer.

Applications for Emerin Antibody (CL0201) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Emerin

Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.

Alternate Names

emerin, Emery-Dreifuss muscular dystrophy, LEM domain containing 5, STAEDMDLEMD5

Gene Symbol

EMD

Additional Emerin Products

Product Documents for Emerin Antibody (CL0201) - BSA Free

Certificate of Analysis

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Product Specific Notices for Emerin Antibody (CL0201) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Emerin Antibody (CL0201) - BSA Free

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Protocols

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FAQs

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