Emerin Antibody (CL0203) - BSA Free

Novus Biologicals | Catalog # NBP2-52877

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0203

Format

BSA Free
View all Emerin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P50402, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Emerin Antibody (CL0203) - BSA Free

Western Blot: Emerin Antibody (CL0203) [NBP2-52877]

Western Blot: Emerin Antibody (CL0203) [NBP2-52877]

Western Blot: Emerin Antibody (CL0203) [NBP2-52877] - Analysis of extracts from RT-4 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-EMD monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity
Immunocytochemistry/ Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877]

Immunocytochemistry/ Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877]

Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877] - Staining in U2OS cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877]

Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877]

Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877] - Staining of human testis shows nuclear membrane immunoreactivity.
Western Blot: Emerin Antibody (CL0203) [NBP2-52877]

Western Blot: Emerin Antibody (CL0203) [NBP2-52877]

Western Blot: Emerin Antibody (CL0203) [NBP2-52877] - Lane 1: Marker [kDa], Lane 2: RT-4
Immunocytochemistry/ Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877]

Immunocytochemistry/ Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877]

Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877] - Staining in A431 cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877]

Immunocytochemistry/ Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877]

Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877] - Staining in HeLa cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877]

Immunocytochemistry/ Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877]

Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877] - Staining in MCF7 cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877]

Immunocytochemistry/ Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877]

Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877] - Staining in U251 cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877]

Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877]

Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877] - Staining of human kidney shows nuclear membrane positivity in renal tubules.
Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877]

Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877]

Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877] - Staining of human placenta shows strong nuclear membrane positivity in the trophoblast.
Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877]

Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877]

Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877] - Staining of human prostate shows strong nuclear membrane immunoreactivityy in glandular cells.
Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877]

Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877]

Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877] - Staining of human skin shows strong nuclear membrane positivity in the epidermis cells.

Applications for Emerin Antibody (CL0203) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Emerin

Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.

Alternate Names

emerin, Emery-Dreifuss muscular dystrophy, LEM domain containing 5, STAEDMDLEMD5

Gene Symbol

EMD

Additional Emerin Products

Product Documents for Emerin Antibody (CL0203) - BSA Free

Certificate of Analysis

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Product Specific Notices for Emerin Antibody (CL0203) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Emerin Antibody (CL0203) - BSA Free

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Protocols

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