Emerin Antibody

Novus Biologicals | Catalog # NBP2-16326

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Emerin Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Emerin. The exact sequence is proprietary.

Localization

Nucleus inner membrane; Single-pass membrane protein; Nucleoplasmic side

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Emerin Antibody

Western Blot: Emerin Antibody [NBP2-16326]

Western Blot: Emerin Antibody [NBP2-16326]

Western Blot: Emerin Antibody [NBP2-16326] - Wild-type (WT) and Emerin knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Emerin antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Emerin Antibody [NBP2-16326]

Immunocytochemistry/ Immunofluorescence: Emerin Antibody [NBP2-16326]

Immunocytochemistry/Immunofluorescence: Emerin Antibody [NBP2-16326] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Emerin Antibody [NBP2-16326]

Immunohistochemistry-Paraffin: Emerin Antibody [NBP2-16326]

Immunohistochemistry-Paraffin: Emerin Antibody [NBP2-16326] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:1000 dilution.
Western Blot: Emerin Antibody [NBP2-16326]

Western Blot: Emerin Antibody [NBP2-16326]

Western Blot: Emerin Antibody [NBP2-16326] - Sample (30 ug of whole cell lysate) A: HeLa nucleus 12% SDS PAGE gel, diluted at 1:5000.

Applications for Emerin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Emerin

Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene. [provided by RefSeq]

Alternate Names

emerin, Emery-Dreifuss muscular dystrophy, LEM domain containing 5, STAEDMDLEMD5

Gene Symbol

EMD

UniProt

P50402 (Human)

Additional Emerin Products

Product Documents for Emerin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Emerin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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