EMILIN1 Antibody (C11A8) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-36338
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2a Kappa Clone # C11A8
Format
Azide and BSA Free
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Product Specifications
Immunogen
recombinant mouse Emilin-1
Clonality
Monoclonal
Host
Rat
Isotype
IgG2a Kappa
Scientific Data Images for EMILIN1 Antibody (C11A8) - Azide and BSA Free
Western Blot: EMILIN1 Antibody (C11A8)Azide and BSA Free [NBP2-36338]
Western Blot: EMILIN1 Antibody (C11A8) [NBP2-36338] - Western blot on recombinant protein obtained from supernatant of cells expressing human EMILIN1 (hE1), mouse EMILIN1 (mE1), mouse EMILIN2 (mE2).Immunocytochemistry/ Immunofluorescence: EMILIN1 Antibody (C11A8) - Azide and BSA Free [NBP2-36338]
Immunocytochemistry/Immunofluorescence: EMILIN1 Antibody (C11A8) [NBP2-36338] - Positive staining of EMILIN1 (green) in mouse lymphatic endothelial cells (LAEC). Nuclei are stained in blue. Scale bar 38 um.Immunohistochemistry-Frozen: EMILIN1 Antibody (C11A8) - Azide and BSA Free [NBP2-36338]
Immunohistochemistry-Frozen: EMILIN1 Antibody (C11A8) [NBP2-36338] - EMILIN1 is expressed in association with lymphatic vessels. Cryostat sections of normal mouse tissues stained with anti-EMILIN1 (green) antibodies. In all mouse tissues and organs examined, EMILIN1 was uniformly distributed in the stroma. In the skin, EMILIN1 staining colocalizes with LYVE-1-positive lymphatic vessels surrounding hair follicles. In the small intestine, EMILIN1 colocalizes with LYVE-1-positive lacteals and submucosal lymphatic vessels. At higher magnification, in the lung and lymph nodes, it is more evident that EMILIN1 is distributed at the abluminal surfaces of LECs. In the lymph node, EMILIN1-positive fibers connecting LECs to the surrounding ECM are evident.Immunocytochemistry/ Immunofluorescence: EMILIN1 Antibody (C11A8) - Azide and BSA Free [NBP2-36338]
Immunocytochemistry/Immunofluorescence: EMILIN1 Antibody (C11A8) [NBP2-36338] - Positive staining for EMILIN1 (green) in mouse lung tissue is both detected in lung parenchymal fibers and associated with lymphatic vessel structures. Lyve-1 (red) is used as lymphatic vessel marker. Blue, nuclei.Scale bar: 37.00 umImmunocytochemistry/ Immunofluorescence: EMILIN1 Antibody (C11A8) - Azide and BSA Free [NBP2-36338]
Immunocytochemistry/Immunofluorescence: EMILIN1 Antibody (C11A8) [NBP2-36338] - Positive staining for EMILIN1 (green) in mouse lymph node. In red, lymphatic vessels positive for Lyve-1. Yellow represents the close association of EMILIN1 with lymphatic vessel structures. Scale bar: 37.00 umImmunocytochemistry/ Immunofluorescence: EMILIN1 Antibody (C11A8) - Azide and BSA Free [NBP2-36338]
Immunocytochemistry/Immunofluorescence: EMILIN1 Antibody (C11A8) [NBP2-36338] - Positive staining for EMILIN1 (green) in mouse lymph node. In red, lymphatic vessels positive for Lyve-1. Yellow represents the close association of EMILIN1 with lymphatic vessel structures. Scale bar: 37.00 um Positive staining for EMILIN1 (red) in mouse skin. Blue, nuclei; Scale bar: 37.00 umImmunocytochemistry/ Immunofluorescence: EMILIN1 Antibody (C11A8) - Azide and BSA Free [NBP2-36338]
Immunocytochemistry/Immunofluorescence: EMILIN1 Antibody (C11A8) [NBP2-36338] - Positive staining of EMILIN1 (green) in mouse fibroblasts (NIH 3T3).cytoskeleton is stained in red, nuclei in blue. Scale bar 28 um.Applications for EMILIN1 Antibody (C11A8) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Western Blot
2-10 ug/ml
Formulation, Preparation, and Storage
Purification
Protein G purified
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: EMILIN1
Alternate Names
DKFZp586M121, elastin microfibril interface-located protein 1, elastin microfibril interfacer 1, EMI, EMILIN, EMILIN-1, gp115
Gene Symbol
EMILIN1
Additional EMILIN1 Products
Product Documents for EMILIN1 Antibody (C11A8) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for EMILIN1 Antibody (C11A8) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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