EMILIN1 Antibody (C11A8) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-36338

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # C11A8

Format

Azide and BSA Free
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Product Specifications

Immunogen

recombinant mouse Emilin-1

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for EMILIN1 Antibody (C11A8) - Azide and BSA Free

Western Blot: EMILIN1 Antibody (C11A8)Azide and BSA Free [NBP2-36338]

Western Blot: EMILIN1 Antibody (C11A8)Azide and BSA Free [NBP2-36338]

Western Blot: EMILIN1 Antibody (C11A8) [NBP2-36338] - Western blot on recombinant protein obtained from supernatant of cells expressing human EMILIN1 (hE1), mouse EMILIN1 (mE1), mouse EMILIN2 (mE2).
Immunocytochemistry/ Immunofluorescence: EMILIN1 Antibody (C11A8) - Azide and BSA Free [NBP2-36338]

Immunocytochemistry/ Immunofluorescence: EMILIN1 Antibody (C11A8) - Azide and BSA Free [NBP2-36338]

Immunocytochemistry/Immunofluorescence: EMILIN1 Antibody (C11A8) [NBP2-36338] - Positive staining of EMILIN1 (green) in mouse lymphatic endothelial cells (LAEC). Nuclei are stained in blue. Scale bar 38 um.
Immunohistochemistry-Frozen: EMILIN1 Antibody (C11A8) - Azide and BSA Free [NBP2-36338]

Immunohistochemistry-Frozen: EMILIN1 Antibody (C11A8) - Azide and BSA Free [NBP2-36338]

Immunohistochemistry-Frozen: EMILIN1 Antibody (C11A8) [NBP2-36338] - EMILIN1 is expressed in association with lymphatic vessels. Cryostat sections of normal mouse tissues stained with anti-EMILIN1 (green) antibodies. In all mouse tissues and organs examined, EMILIN1 was uniformly distributed in the stroma. In the skin, EMILIN1 staining colocalizes with LYVE-1-positive lymphatic vessels surrounding hair follicles. In the small intestine, EMILIN1 colocalizes with LYVE-1-positive lacteals and submucosal lymphatic vessels. At higher magnification, in the lung and lymph nodes, it is more evident that EMILIN1 is distributed at the abluminal surfaces of LECs. In the lymph node, EMILIN1-positive fibers connecting LECs to the surrounding ECM are evident.
Immunocytochemistry/ Immunofluorescence: EMILIN1 Antibody (C11A8) - Azide and BSA Free [NBP2-36338]

Immunocytochemistry/ Immunofluorescence: EMILIN1 Antibody (C11A8) - Azide and BSA Free [NBP2-36338]

Immunocytochemistry/Immunofluorescence: EMILIN1 Antibody (C11A8) [NBP2-36338] - Positive staining for EMILIN1 (green) in mouse lung tissue is both detected in lung parenchymal fibers and associated with lymphatic vessel structures. Lyve-1 (red) is used as lymphatic vessel marker. Blue, nuclei.Scale bar: 37.00 um
Immunocytochemistry/ Immunofluorescence: EMILIN1 Antibody (C11A8) - Azide and BSA Free [NBP2-36338]

Immunocytochemistry/ Immunofluorescence: EMILIN1 Antibody (C11A8) - Azide and BSA Free [NBP2-36338]

Immunocytochemistry/Immunofluorescence: EMILIN1 Antibody (C11A8) [NBP2-36338] - Positive staining for EMILIN1 (green) in mouse lymph node. In red, lymphatic vessels positive for Lyve-1. Yellow represents the close association of EMILIN1 with lymphatic vessel structures. Scale bar: 37.00 um
Immunocytochemistry/ Immunofluorescence: EMILIN1 Antibody (C11A8) - Azide and BSA Free [NBP2-36338]

Immunocytochemistry/ Immunofluorescence: EMILIN1 Antibody (C11A8) - Azide and BSA Free [NBP2-36338]

Immunocytochemistry/Immunofluorescence: EMILIN1 Antibody (C11A8) [NBP2-36338] - Positive staining for EMILIN1 (green) in mouse lymph node. In red, lymphatic vessels positive for Lyve-1. Yellow represents the close association of EMILIN1 with lymphatic vessel structures. Scale bar: 37.00 um Positive staining for EMILIN1 (red) in mouse skin. Blue, nuclei; Scale bar: 37.00 um
Immunocytochemistry/ Immunofluorescence: EMILIN1 Antibody (C11A8) - Azide and BSA Free [NBP2-36338]

Immunocytochemistry/ Immunofluorescence: EMILIN1 Antibody (C11A8) - Azide and BSA Free [NBP2-36338]

Immunocytochemistry/Immunofluorescence: EMILIN1 Antibody (C11A8) [NBP2-36338] - Positive staining of EMILIN1 (green) in mouse fibroblasts (NIH 3T3).cytoskeleton is stained in red, nuclei in blue. Scale bar 28 um.

Applications for EMILIN1 Antibody (C11A8) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

2-10 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EMILIN1

The EMILIN1 gene encodes an extracellular matrix glycoprotein that is characterized by an N-terminal microfibril interfacedomain, a coiled-coiled alpha-helical domain, a collagenous domain and a C-terminal globular C1q domain. The encodedprotein associates with

Alternate Names

DKFZp586M121, elastin microfibril interface-located protein 1, elastin microfibril interfacer 1, EMI, EMILIN, EMILIN-1, gp115

Gene Symbol

EMILIN1

Additional EMILIN1 Products

Product Documents for EMILIN1 Antibody (C11A8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for EMILIN1 Antibody (C11A8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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