Enah/Vasp-like Antibody (5G1) - Azide and BSA Free

Novus Biologicals | Catalog # H00051466-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5G1

Format

Azide and BSA Free
View all Enah/Vasp-like Primary Antibodies »

Product Specifications

Immunogen

EVL (NP_057421, 309 a.a. ~ 418 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PGTRAASQPPNSSEAGRKPWERSNSVEKPVSSILSRTPSVAKSPEAKSPLQSQPHSRMKPAGSVNDMALDAFDLDRMKQEILEEVVRELHKVKEEIIDAIRQELSGISTT

Reactivity Notes

Human. Other species not tested.

Localization

Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Note=Targeted to the leading edge of lamellipodia and the distal tip of stress fibers through interaction with a number of proteins. In activated T-cells, localizes to the F-actin collar and the dis

Specificity

EVL - Enah/Vasp-like

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Enah/Vasp-like Antibody (5G1) - Azide and BSA Free

Western Blot: Enah/Vasp-like Antibody (5G1) [H00051466-M01]

Western Blot: Enah/Vasp-like Antibody (5G1) [H00051466-M01]

Western Blot: Enah/Vasp-like Antibody (5G1) [H00051466-M01] - Analysis of EVL expression in transfected 293T cell line by EVL monoclonal antibody (M01), clone 5G1.Lane 1: EVL transfected lysate(45 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Enah/Vasp-like Antibody (5G1) [H00051466-M01]

Immunocytochemistry/ Immunofluorescence: Enah/Vasp-like Antibody (5G1) [H00051466-M01]

Immunocytochemistry/Immunofluorescence: Enah/Vasp-like Antibody (5G1) [H00051466-M01] - Analysis of monoclonal antibody to EVL on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: Enah/Vasp-like Antibody (5G1) [H00051466-M01] - Detection limit for recombinant GST tagged EVL is approximately 0.03ng/ml as a capture antibody.

Applications for Enah/Vasp-like Antibody (5G1) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA immunofluorescence.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Enah/Vasp-like

Enah/Vasp-like (EVL) is a member of the Ena/VASP protein family. Ena/VASP proteins are actin-associated proteins involved in a range of processes that require dynamic actin remodeling such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. EVL enhances actin nucleation and polymerization.

Alternate Names

ena/VASP-like protein, Enah/Vasp-like, RNB6Ena/vasodilator-stimulated phosphoprotein-like

Entrez Gene IDs

51466 (Human)

Gene Symbol

EVL

UniProt

NP_057421 (Human)

Additional Enah/Vasp-like Products

Product Documents for Enah/Vasp-like Antibody (5G1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Enah/Vasp-like Antibody (5G1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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