engrailed homeobox 2 Antibody

Novus Biologicals | Catalog # NB100-2814

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Engrailed-2 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-NHSTTAKEGKSDSE corresponding to C-Terminus according to NP_001418.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for engrailed homeobox 2 Antibody

Western Blot: engrailed homeobox 2 Antibody [NB100-2814]

Western Blot: engrailed homeobox 2 Antibody [NB100-2814]

Western Blot: engrailed homeobox 2 Antibody [NB100-2814] - Western blot analysis of Mouse Brain lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: engrailed homeobox 2 Antibody [NB100-2814]

Immunocytochemistry/ Immunofluorescence: engrailed homeobox 2 Antibody [NB100-2814]

Immunocytochemistry/Immunofluorescence: engrailed homeobox 2 Antibody [NB100-2814] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunocytochemistry/ Immunofluorescence: engrailed homeobox 2 Antibody [NB100-2814]

Immunocytochemistry/ Immunofluorescence: engrailed homeobox 2 Antibody [NB100-2814]

Immunocytochemistry/Immunofluorescence: engrailed homeobox 2 Antibody [NB100-2814] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Applications for engrailed homeobox 2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:4000

Western Blot

0.1 - 1 ug/mL
Application Notes
Western blot: Approx 35kDa band observed in Mouse Brain lysates (calculated MW of f 33.8kDa according to Mouse NP_034264.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Engrailed-2

EN1 and EN2 encode homeodomain containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. EN2 may play a role in susceptibility to autism spectrum disorders.

Alternate Names

EN2, Engrailed2

Entrez Gene IDs

2020 (Human); 13799 (Mouse); 499964 (Rat)

Gene Symbol

EN2

UniProt

P19622

Additional Engrailed-2 Products

Product Documents for engrailed homeobox 2 Antibody

Certificate of Analysis

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Product Specific Notices for engrailed homeobox 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for engrailed homeobox 2 Antibody

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Protocols

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