Enolase 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31324

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Predicted:

Bovine (93%), Chicken (93%), Chimpanzee (100%), Guinea Pig (95%), Rhesus Macaque (100%), Xenopus (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Dot Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Enolase 1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Enolase 1. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (90%).

Localization

Cytoplasm, Cell membrane, myofibril, sarcomere, M-band, Isoform MBP-1: Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Enolase 1 Antibody - BSA Free

Western Blot: Enolase 1 Antibody [NBP1-31324]

Western Blot: Enolase 1 Antibody [NBP1-31324]

Western Blot: Enolase 1 Antibody [NBP1-31324] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Enolase 1 Antibody [NBP1-31324]

Immunocytochemistry/ Immunofluorescence: Enolase 1 Antibody [NBP1-31324]

Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody [NBP1-31324] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ENO1 protein stained by ENO1 antibody [N3C3] diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Enolase 1 Antibody [NBP1-31324]

Immunohistochemistry-Paraffin: Enolase 1 Antibody [NBP1-31324]

Immunohistochemistry-Paraffin: Enolase 1 Antibody [NBP1-31324] - Paraffin-embedded gastric tissue, using antibody at 1:100 dilution.
Western Blot: Enolase 1 Antibody [NBP1-31324]

Western Blot: Enolase 1 Antibody [NBP1-31324]

Western Blot: Enolase 1 Antibody [NBP1-31324] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.
Western Blot: Enolase 1 Antibody [NBP1-31324]

Western Blot: Enolase 1 Antibody [NBP1-31324]

Western Blot: Enolase 1 Antibody [NBP1-31324] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 10% SDS PAGE, antibody diluted at 1:3000.
Western Blot: Enolase 1 Antibody [NBP1-31324]

Western Blot: Enolase 1 Antibody [NBP1-31324]

Western Blot: Enolase 1 Antibody [NBP1-31324] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3].
Western Blot: Enolase 1 Antibody [NBP1-31324]

Western Blot: Enolase 1 Antibody [NBP1-31324]

Western Blot: Enolase 1 Antibody [NBP1-31324] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Enolase 1 Antibody

Western Blot: Enolase 1 Antibody [NBP1-31324] -

Western Blot: Enolase 1 Antibody [NBP1-31324] - Sample (30 ug of whole cell lysate)
A: zebrafish eye
10% SDS PAGE
NBP1-31324 diluted at 1:1000
Enolase 1 Antibody

Immunohistochemistry: Enolase 1 Antibody [NBP1-31324] -

Immunohistochemistry: Enolase 1 Antibody [NBP1-31324] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Enolase 1 antibody [N3C3] (NBP1-31324) at 1:300 dilution.
Enolase 1 Antibody

Immunohistochemistry: Enolase 1 Antibody [NBP1-31324] -

Immunohistochemistry: Enolase 1 Antibody [NBP1-31324] - Enolase 1 antibody [N3C3] detects Eno1 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Enolase 1 antibody [N3C3] (NBP1-31324) dilution: 1:100.

Applications for Enolase 1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:20000
Application Notes
Dot Blot-Assay dependent

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Enolase 1

This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. [provided by RefSeq]

Alternate Names

2-phospho-D-glycerate hydro-lyase, Alpha enolase, C-myc promoter-binding protein, c-myc promoter-binding protein-1, EC 4.2.1, EC 4.2.1.11, ENO1L1, Enolase 1, enolase 1, (alpha), MBP-1, MBPB1, MPB-1, MPB1c-myc promoter-binding protein 1, MYC promoter-binding protein 1, NNE, Non-neural enolase, Phosphopyruvate hydratase, Plasminogen-binding protein, PPHalpha enolase like 1, tau-crystallin

Gene Symbol

ENO1

Additional Enolase 1 Products

Product Documents for Enolase 1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Enolase 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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