Enolase 1 Antibody

Novus Biologicals | Catalog # NBP2-16337

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (96%), Chicken (92%), Guinea Pig (95%), Primate (100%), Rhesus Macaque (100%), Xenopus (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Enolase 1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Enolase 1. The exact sequence is proprietary.

Reactivity Notes

Chimpanzee (100%), Xenopus laevis (92%).

Localization

Cytoplasm; Cell membrane; myofibril; sarcomere; M-band; Isoform MBP-1: Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Enolase 1 Antibody

Western Blot: Enolase 1 Antibody [NBP2-16337]

Western Blot: Enolase 1 Antibody [NBP2-16337]

Western Blot: Enolase 1 Antibody [NBP2-16337] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Enolase 1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Enolase 1 Antibody [NBP2-16337]

Immunocytochemistry/ Immunofluorescence: Enolase 1 Antibody [NBP2-16337]

Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody [NBP2-16337] - Immunofluorescence analysis of methanol-fixed HCT116, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Enolase 1 Antibody [NBP2-16337]

Immunohistochemistry-Paraffin: Enolase 1 Antibody [NBP2-16337]

Immunohistochemistry-Paraffin: Enolase 1 Antibody [NBP2-16337] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.
Western Blot: Enolase 1 Antibody [NBP2-16337]

Western Blot: Enolase 1 Antibody [NBP2-16337]

Western Blot: Enolase 1 Antibody [NBP2-16337] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody.
Western Blot: Enolase 1 Antibody [NBP2-16337]

Western Blot: Enolase 1 Antibody [NBP2-16337]

Western Blot: Enolase 1 Antibody [NBP2-16337] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Enolase 1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunoprecipitation: Enolase 1 Antibody [NBP2-16337]

Immunoprecipitation: Enolase 1 Antibody [NBP2-16337]

Immunoprecipitation: Enolase 1 Antibody [NBP2-16337] - Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2. 5 ug of preimmune rabbit IgG C. Immunoprecipitation of ENO1 protein by 2. 5 ug of ENO1 antibody 10% SDS-PAGE The immunoprecipitated ENO1 protein was detected by ENO1 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.
Enolase 1 Antibody

Western Blot: Enolase 1 Antibody [NBP2-16337] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody (NBP2-16337) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Enolase 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:500-1:1000

Western Blot

1:5000-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Enolase 1

This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. [provided by RefSeq]

Alternate Names

2-phospho-D-glycerate hydro-lyase, Alpha enolase, C-myc promoter-binding protein, c-myc promoter-binding protein-1, EC 4.2.1, EC 4.2.1.11, ENO1L1, Enolase 1, enolase 1, (alpha), MBP-1, MBPB1, MPB-1, MPB1c-myc promoter-binding protein 1, MYC promoter-binding protein 1, NNE, Non-neural enolase, Phosphopyruvate hydratase, Plasminogen-binding protein, PPHalpha enolase like 1, tau-crystallin

Gene Symbol

ENO1

UniProt

P06733 (Human)

Additional Enolase 1 Products

Product Documents for Enolase 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Enolase 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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