Enolase 2/Neuron-specific Enolase Antibody (1C1) - BSA Free

Novus Biologicals | Catalog # NB200-422

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1C1

Format

BSA Free
View all Enolase 2/Neuron-specific Enolase Primary Antibodies »

Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified NSE from human brain.

Localization

Cytoplasmic

Marker

Neuronal Marker

Specificity

Human NSE, gamma-gamma subunits

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Enolase 2/Neuron-specific Enolase Antibody (1C1) - BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended pairs for sandwich immunoassay (coating - conjugate): NB200-421 - NB200-422, NB200-422 - NB200-421.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50 mM sodium citrate, 150 mM NaCl, pH 6.0

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Enolase 2/Neuron-specific Enolase

Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (alpha, beta and gamma). The alpha subunit is expressed in most tissues and the beta subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. NSE (neuron specific enolase) is found in elevated concentrations in plasma and certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.

Alternate Names

ENO2, gamma-Enolase, Neuronspecific Enolase, NSE

Entrez Gene IDs

2026 (Human)

Gene Symbol

ENO2

Additional Enolase 2/Neuron-specific Enolase Products

Product Documents for Enolase 2/Neuron-specific Enolase Antibody (1C1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Enolase 2/Neuron-specific Enolase Antibody (1C1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Neuronal Markers

Citations for Enolase 2/Neuron-specific Enolase Antibody (1C1) - BSA Free

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Protocols

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FAQs

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