Enolase 2/Neuron-specific Enolase Antibody (NSEP2) - BSA Free

Novus Biologicals | Catalog # NBP2-50533

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # NSEP2

Format

BSA Free
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Product Specifications

Immunogen

Ovalbumin-conjugated synthetic peptides corresponding to human NSE amino acid sequence:NSE-P2: aa's 271-285 - TGDQLGALYQDFVRD

Specificity

Not reactive with -isozyme of enolase.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Enolase 2/Neuron-specific Enolase Antibody (NSEP2) - BSA Free

Western Blot: Enolase 2/Neuron-specific Enolase Antibody (NSEP2)BSA Free [NBP2-50533]

Western Blot: Enolase 2/Neuron-specific Enolase Antibody (NSEP2)BSA Free [NBP2-50533]

Western Blot: Enolase 2/Neuron-specific Enolase Antibody (NSEP2) [NBP2-50533] - Lysates were separated on SDS-PAGE and probed with Anti-NSE [NSE-P2]. Lane 1: SH-SY5Y, Lane 2: NT2/D1.

Applications for Enolase 2/Neuron-specific Enolase Antibody (NSEP2) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s): Formalin-fixed, paraffin-embedded nerve tissue sections

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Enolase 2/Neuron-specific Enolase

Neuron-specific enolase (NSE) is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release NSE into the blood. NSE is a useful substance that has been detected in patients with certain tumors, namely: neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma. Recombinant NSE was expressed in E.coli and purified by conventional chromatography techniques.

Alternate Names

ENO2, gamma-Enolase, Neuronspecific Enolase, NSE

Gene Symbol

ENO2

Additional Enolase 2/Neuron-specific Enolase Products

Product Documents for Enolase 2/Neuron-specific Enolase Antibody (NSEP2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Enolase 2/Neuron-specific Enolase Antibody (NSEP2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Enolase 2/Neuron-specific Enolase Antibody (NSEP2) - BSA Free

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  • Q: Enolase came up as a candidate in mass spec. There are at least three isoforms which showed up once or twice or three times (as in three mass spec repeats). To validate the mass data, we are looking into quantitate each isoform. Which antibodies are specific for each of the three?

    A: We checked all antibodies to Enolase 1, Enolase 2 and Enolase 3, but unfortunately no cross reactivity testing was performed for any of these antibodies. Considering how similar these proteins are, it is reasonable to expect some of them will react with other enolases. Base on immunogen sequence, we found some that were raised to the most diverse regions between these proteins, so it is possible that these will be the most  specific to the protein they were raised to.

    For Enolase 1, your best bet is NBP2-25147  as it was raised to N terminal peptide of bovine Eno1: MSILKVHAREIF

    For Enolase 2, I found 3 candidate antibodies:
    NBP2-53158  and NBP2-50532  were raised to aa416-433 (ELGDEARFAGHNFRNPSVL) - it says these antibodies are only reactive with human but they will also work for mouse since the immunogen is 100% match
    NBP2-50533, which was raised to aa271-285 (GDQLGALYQDFVRD) - which is also likely to react with mouse, the immunogen is 93% match with mouse (only last aminoacid is different)

    For Enolase 3, your best bet would be H00002027-M01 or H00002027-A01. both were raised to aa228-277 (KTAIQAAGYPDKVVIGMDVAASEFYRNGKYDLDFKSPDDPARHITGEKLG) - 98% match to mouse

    The immunogens for all these antibodies fall into most variable regions, however, since we have not performed crossreactivity testing, we can not guarantee that they will not crossreact to some extent.

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