eNOS Antibody (6H2) - BSA Free
Novus Biologicals | Catalog # NBP1-51582
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 6H2
Format
BSA Free
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Product Specifications
Immunogen
Purified recombinant fragment of human eNOS expressed in E. coli. [UniProt# P29474]
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
133 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Mouse eNOS Antibody (6H2) - BSA Free (NBP1-51582) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-eNOS Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for eNOS Antibody (6H2) - BSA Free
![Western Blot: eNOS Antibody (6H2)BSA Free [NBP1-51582]](https://beta-resources.rndsystems.com/images/products/eNOS-Antibody-6H2-Western-Blot-NBP1-51582-img0003.jpg "Western Blot: eNOS Antibody (6H2)BSA Free [NBP1-51582]")
Western Blot: eNOS Antibody (6H2)BSA Free [NBP1-51582]
Western Blot: eNOS Antibody (6H2) [NBP1-51582] - Western blot analysis of HUVEC cells (A) using eNOS antibody at a concentration of 2 ug/ml.![Immunohistochemistry-Paraffin: eNOS Antibody (6H2) - BSA Free [NBP1-51582]](https://beta-resources.rndsystems.com/images/products/eNOS-Antibody-6H2-Immunohistochemistry-Paraffin-NBP1-51582-img0004.jpg "Immunohistochemistry-Paraffin: eNOS Antibody (6H2) - BSA Free [NBP1-51582]")
Immunohistochemistry-Paraffin: eNOS Antibody (6H2) - BSA Free [NBP1-51582]
Immunohistochemistry-Paraffin: eNOS Antibody (6H2) [NBP1-51582] - IHC staining of eNOS in human placenta using DAB with hematoxylin counterstain.![Immunohistochemistry-Paraffin: eNOS Antibody (6H2) - BSA Free [NBP1-51582]](https://beta-resources.rndsystems.com/images/products/eNOS-Antibody-6H2-Immunohistochemistry-Paraffin-NBP1-51582-img0001.jpg "Immunohistochemistry-Paraffin: eNOS Antibody (6H2) - BSA Free [NBP1-51582]")
Immunohistochemistry-Paraffin: eNOS Antibody (6H2) - BSA Free [NBP1-51582]
Immunohistochemistry-Paraffin: eNOS Antibody (6H2) [NBP1-51582] - Immunohistochemical analysis of paraffin-embedded human lymph node (left) and colon cancer (right) tissues using eNOS mouse mAb with DAB staining.![Immunohistochemistry-Paraffin: eNOS Antibody (6H2) - BSA Free [NBP1-51582]](https://beta-resources.rndsystems.com/images/products/eNOS-Antibody-6H2-Immunohistochemistry-Paraffin-NBP1-51582-img0002.jpg "Immunohistochemistry-Paraffin: eNOS Antibody (6H2) - BSA Free [NBP1-51582]")
Immunohistochemistry-Paraffin: eNOS Antibody (6H2) - BSA Free [NBP1-51582]
Immunohistochemistry-Paraffin: eNOS Antibody (6H2) [NBP1-51582] - Immunohistochemical analysis of paraffin-embedded human stomach cancer (left) and ovary cancer (right) tissues using eNOS mouse mAb with DAB staining.Applications for eNOS Antibody (6H2) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Immunohistochemistry
1:100
Immunohistochemistry-Paraffin
1:100
Western Blot
2 ug/ml
Application Notes
This eNOS (6H2) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections and ELISA.
Formulation, Preparation, and Storage
Purification
Ammonium sulfate precipitation
Formulation
PBS
Format
BSA Free
Preservative
0.03% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: eNOS
Long Name
Endothelial Nitric Oxide Synthase
Alternate Names
NOS3
Entrez Gene IDs
4846 (Human)
Gene Symbol
NOS3
UniProt
Additional eNOS Products
Product Documents for eNOS Antibody (6H2) - BSA Free
Product Specific Notices for eNOS Antibody (6H2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for eNOS Antibody (6H2) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
