ENPP-5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86449

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ENPP-5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KFWEEATPIWITNQRAGHTSGAAMWPGTDVKIHKRFPTHYMPYNESVSFEDRVAKIIEWFTSKEPINLGLLYWEDPDDMGHHLGPDSPLMGPVISDIDKKLGYLIQMLKKAKLWNTLNLIITSDHGMTQCSE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ENPP-5 Antibody - BSA Free

ENPP-5 Antibody - BSA Free Immunohistochemistry-Paraffin: ENPP-5 Antibody - BSA Free [NBP1-86449]

Immunohistochemistry-Paraffin: ENPP-5 Antibody - BSA Free [NBP1-86449]

Analysis in human epididymis and liver tissues using Anti-ENPP5 antibody. Corresponding ENPP5 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ENPP-5 Antibody [NBP1-86449]

Immunohistochemistry-Paraffin: ENPP-5 Antibody [NBP1-86449]

Immunohistochemistry-Paraffin: ENPP-5 Antibody [NBP1-86449] - Staining of human epididymis shows high expression.
Immunohistochemistry-Paraffin: ENPP-5 Antibody [NBP1-86449]

Immunohistochemistry-Paraffin: ENPP-5 Antibody [NBP1-86449]

Immunohistochemistry-Paraffin: ENPP-5 Antibody [NBP1-86449] - Staining of human liver shows low expression as expected.
ENPP-5 Antibody - BSA Free Western Blot: ENPP-5 Antibody - BSA Free [NBP1-86449]

Western Blot: ENPP-5 Antibody - BSA Free [NBP1-86449]

Analysis in control (vector only transfected HEK293T lysate) and ENPP5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for ENPP-5 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ENPP-5

Ectonucleotide pyrophosphatase/phosphodiesterase family member 5 (ENPP5) is a member of the nucleotide pyrophosphatase/phosphodiesterase family. Members of the pyrophosphatase/phosphodiesterase family are involved in bone mineralization, purinergic receptor signaling, and cell motility. ENPP5 plays a role in neuronal cell communication and, unlike its other family members, lacks nucleotide pyrophosphatase and lysopholipase D activity.

Long Name

Ectonucleotide Pyrophosphatase/Phosphodiesterase 5

Alternate Names

ENPP5, NPP5

Gene Symbol

ENPP5

Additional ENPP-5 Products

Product Documents for ENPP-5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ENPP-5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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