Enterovirus D68 VP1 Antibody (GT1843) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-13564
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Key Product Details
Species Reactivity
Virus
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Lateral Flow Assay
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # GT1843
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of Enterovirus D68 VP1 protein. The exact sequence is proprietary.
Reactivity Notes
Enterovirus D68
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Enterovirus D68 VP1 Antibody (GT1843) - Azide and BSA Free
Elisa- Enterovirus D68 VP1 Antibody (1843) [NBP3-13564] -
Elisa- Enterovirus D68 VP1 Antibody (1843) [NBP3-13564] -Indirect ELISA analysis was performed by coating the plate with recombinant full-length enterovirus D68 VP1 (4000-62.5 ng/mL). Coated protein was probed with Enterovirus D68 VP1 antibody [1843] (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.
Western Blot: Enterovirus D68 VP1 Antibody (GT1843) [NBP3-13564] -
Western Blot: Enterovirus D68 VP1 Antibody (GT1843) [NBP3-13564] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [1843] diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
ELISA: Enterovirus D68 VP1 Antibody (GT1843) [NBP3-13564] -
ELISA: Enterovirus D68 VP1 Antibody (GT1843) [NBP3-13564] - Sandwich ELISA detection of recombinant full-length VP1 protein(s) derived from different strains of Enterovirus (ie., D68; 71) using antibodies as below.Capture: Enterovirus D68 VP1 antibody [GT1843] (NBP3-13564) (5 ug/mL)
Detection: Enterovirus D68 VP1 antibody [HL1997] (1 ug/mL)
Lateral Flow Assay: Enterovirus D68 VP1 Antibody (GT1843) [NBP3-13564] -
Lateral Flow Assay: Enterovirus D68 VP1 Antibody (GT1843) [NBP3-13564] - Detection of enterovirus D68 VP1 protein by lateral flow assay using the monoclonal antibody pair.Capture: Enterovirus D68 VP1 antibody (NBP3-13564 [GT1843])
Detection: Enterovirus D68 VP1 antibody
Samples (80 ng) :
1. Enterovirus D68 VP1 protein
2. Lysis buffer
Lateral Flow Assay: Enterovirus D68 VP1 Antibody (GT1843) [NBP3-13564] -
Lateral Flow Assay: Enterovirus D68 VP1 Antibody (GT1843) [NBP3-13564] - Detection of enterovirus D68 VP1 protein by lateral flow assay using the monoclonal antibody pair.Capture: Enterovirus D68 VP1 antibody
Detection: Enterovirus D68 VP1 antibody (NBP3-13564 [GT1843])
Samples (80 ng) :
1. Enterovirus D68 VP1 protein
2. Lysis buffer
Immunocytochemistry/ Immunofluorescence: Enterovirus D68 VP1 Antibody (GT1843) [NBP3-13564] -
Enterovirus D68 VP1 antibody [GT1843] detects Enterovirus D68 VP1 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Enterovirus D68 VP1 stained by Enterovirus D68 VP1 antibody [GT1843] (NBP3-13564) diluted at 1:500.
Blue: Fluoroshield with DAPI.
Western Blot: Enterovirus D68 VP1 Antibody (GT1843) [NBP3-13564] -
Non-infected (-) and infected (+) RD whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [GT1843] (NBP3-13564) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Enterovirus D68 VP1 Antibody (GT1843) [NBP3-13564] -
Enterovirus D68 VP1 antibody [GT1843] detects Enterovirus D68 VP1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mock and Enterovirus D68 VP1 transfected 293T cell.
Green: Enterovirus D68 VP1 stained by Enterovirus D68 VP1 antibody [GT1843] (NBP3-13564) diluted at 1:4000.
Blue: Fluoroshield with DAPI.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Applications for Enterovirus D68 VP1 Antibody (GT1843) - Azide and BSA Free
Application
Recommended Usage
ELISA
Assay dependent
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Lateral Flow Assay
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 20% Glycerol
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Enterovirus D68 VP1
Alternate Names
Capsid protein VP1, EV68 VP1, P1D, Virion protein 1
Additional Enterovirus D68 VP1 Products
Product Documents for Enterovirus D68 VP1 Antibody (GT1843) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Enterovirus D68 VP1 Antibody (GT1843) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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