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EOMES Antibody (CL15425) - BSA Free

Novus Biologicals | Catalog # NBP3-43667

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Scientific Data
Applications
Preparation & Storage
Background
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Research Areas

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # CL15425

Format

BSA Free

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Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of O95936, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for EOMES Antibody (CL15425) - BSA Free

Immunohistochemistry-Paraffin: EOMES Antibody (CL15425) [NBP3-43667]

Staining of human prostate shows no positivity in glandular cells as expected.

Immunohistochemistry-Paraffin: EOMES Antibody (CL15425) [NBP3-43667]

Analysis in human tonsil and prostate tissues using NBP3-43667 antibody. Corresponding EOMES RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: EOMES Antibody (CL15425) [NBP3-43667]

Staining of human tonsil shows strong nuclear positivity in non-germinal center cells.

Immunohistochemistry-Paraffin: EOMES Antibody (CL15425) [NBP3-43667]

Staining of mouse brain shows moderate to strong nuclear positivity in a subset of neurons in the cerebellum.

Immunohistochemistry-Paraffin: EOMES Antibody (CL15425) [NBP3-43667]

Staining of human cerebellum shows strong nuclear positivity in a subset of neurons in the granular layer.

Immunohistochemistry-Paraffin: EOMES Antibody (CL15425) [NBP3-43667]

Staining of mouse embryo, E14 shows moderate to strong nuclear positivity in neuronal cells in the developing cortex.

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Applications for EOMES Antibody (CL15425) - BSA Free

Application

Recommended Usage

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EOMES

EOMES encodes a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar gene disrupted in mice is shown to be essential during trophoblast development and gastrulation.

Long Name

Eomesodermin Homolog

Alternate Names

Eomesodermin, TBR2

Gene Symbol

EOMES

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Product Specific Notices for EOMES Antibody (CL15425) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.