EphB4 Antibody (5B5)

Name: EphB4 Antibody (5B5)
Brand: Novus Biologicals
SKU: NBP1-47434
Price: 139 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-47434

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 5B5

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Product Specifications

Immunogen

Purified recombinant extracellular fragment of human EphB4 fused with hIgGFc tag expressed in HEK293 cell line.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

108 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse EphB4 Antibody (5B5) (NBP1-47434) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for EphB4 Antibody (5B5)

Western Blot: EphB4 Antibody (5B5)BSA Free [NBP1-47434]

Western Blot: EphB4 Antibody (5B5) [NBP1-47434] - Western blot analysis using anti-EphB4 monoclonal antibody against extracellular domain of human EphB4(aa16-539).

Immunocytochemistry/ Immunofluorescence: EphB4 Antibody (5B5) - BSA Free [NBP1-47434]

Immunocytochemistry/Immunofluorescence: EphB4 Antibody (5B5) [NBP1-47434] - Confocal immunofluorescence analysis of methanol-fixed HEK293 cells trasfected with EphB4-hIgGFc using anti-EphB4 monoclonal antioby(green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.

Applications for EphB4 Antibody (5B5)

Application

Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1: 500-1:2000

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EphB4

EphB4, also known as Htk, Myk1, Tyro11, and Mdk2, is a member of the Eph receptor family, which binds of the ephrin ligand family. Two classes of receptors exist, designated A and B, that have an extracellular domain made up of a globular domain, a cysteine-rich domain, and two fibronectin type III domains, followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains juxtamembrane motif with two tyrosines, which are the major autophosphorylation sites, along with a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxyl terminus, which includes one conserved tyrosine. Ligand recognition and binding leads to activation of intrinsic kinase activity. Only membrane-bound or Fc-clustered ligands have been shown to activate the receptor in vitro. Soluble monomeric ligands can bind the receptor, but do not induce receptor autophosphorylation and activation. The Eph receptors and ephrin ligands display reciprocal expression in vivo. Developing and adult neural tissue express nearly all of the Eph receptors and ephrin ligands.Ephs and ephrins play a significant role in angiogenesis.

Long Name

Eph Receptor B4

Alternate Names

Htk, Mdk2, Myk1, Tyro11

Gene Symbol

EPHB4

Additional EphB4 Products

Product Documents for EphB4 Antibody (5B5)

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Certificate of Analysis

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Product Specific Notices for EphB4 Antibody (5B5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Ephrin Ligands and Eph Receptors

Receptor Tyrosine Kinases (RTKs)

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