Ephrin-A1 Antibody (5D4Q5)

Name: Ephrin-A1 Antibody (5D4Q5)
Brand: Novus Biologicals
SKU: NBP3-16761
Price: 449 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16761

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5D4Q5 expressed in HEK293

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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Ephrin-A1 (P20827). MEFLWAPLLGLCCSLAAADRHTVFWNSSNPKFRNEDYTIHVQLNDYVDIICPHYEDHSVADAAMEQYILYLVEHEEYQLCQPQSKDQVRWQCNRPSAKHG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Ephrin-A1 Antibody (5D4Q5) (NBP3-16761) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Ephrin-A1 Antibody (5D4Q5)

Western Blot: Ephrin-A1 Antibody (5D4Q5) [NBP3-16761]

Western Blot: Ephrin-A1 Antibody (5D4Q5) [NBP3-16761] - Analysis of extracts of HepG2 cells, using Ephrin-A1 antibody (NBP3-16761) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunocytochemistry/ Immunofluorescence: Ephrin-A1 Antibody (5D4Q5) [NBP3-16761]

Immunocytochemistry/Immunofluorescence: Ephrin-A1 Antibody (5D4Q5) [NBP3-16761] - Immunofluorescence analysis of NIH-3T3 cells using Ephrin-A1 Rabbit mAb (NBP3-16761) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: Ephrin-A1 Antibody (5D4Q5) [NBP3-16761]

Immunohistochemistry-Paraffin: Ephrin-A1 Antibody (5D4Q5) [NBP3-16761] - Human brain using Ephrin-A1 Rabbit mAb (NBP3-16761) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence: Ephrin-A1 Antibody (5D4Q5) [NBP3-16761] -

Immunocytochemistry/ Immunofluorescence: Ephrin-A1 Antibody (5D4Q5) [NBP3-16761] - Immunofluorescence analysis of NIH-3T3 cells using Ephrin-A1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: Ephrin-A1 Antibody (5D4Q5) [Ephrin-A1] -

Immunocytochemistry/ Immunofluorescence: Ephrin-A1 Antibody (5D4Q5) [Ephrin-A1] - Immunofluorescence analysis of NIH-3T3 cells using Ephrin-A1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Applications for Ephrin-A1 Antibody (5D4Q5)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 -1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ephrin-A1

The Eph subfamily represents the largest group of receptor protein kinases identified to date. There is increasing evidence that Eph family members are involved in central nervous system function and in development. Ligands for Eph receptors include ephrin-A1 (LERK-1/B61), identified as a ligand for the EphA2 (Eck) receptor; ephrin-A2 (ELF-1), identified as a ligand for the EphA3 and EphA4 (Sek) receptors; ephrin-A3 (LERK-3), identified as a ligand for EphA5 (Ehk1) and EphA3 (Hek) receptors; ephrin-A4 (LERK-4), identified as a ligand for the EphA3 receptor; ephrin-A5 (AL-1), identified as a ligand for EphA5 (REK7); ephrin-B1 (LERK-2), identified as a ligand for the EphB1 (Elk) and EphB2 (Cek5) receptors; ephrin-B2 (LERK-5), identified as a ligand for the EphB1, EphB3 (Cek10) and EphB2 receptors; and ephrin-B3 (LERK-8), identified as a ligand for EphB1.

Alternate Names

B61, EFL-1, EFNA1, EphrinA1, LERK1

Gene Symbol

EFNA1

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Product Documents for Ephrin-A1 Antibody (5D4Q5)

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Product Specific Notices for Ephrin-A1 Antibody (5D4Q5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Ephrin Ligands and Eph Receptors

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