Ephrin-B2 Antibody

Novus Biologicals | Catalog # NBP1-49857

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Predicted:

Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Ephrin-B2 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence CPKVDSKTVGQYE corresponding to internal region according to NP_004084.1.

Epitope

CPKVDSKTVGQYE - internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Ephrin-B2 Antibody

Western Blot: Ephrin-B2 Antibody [NBP1-49857]

Western Blot: Ephrin-B2 Antibody [NBP1-49857]

Western Blot: Ephrin B2 Antibody [NBP1-49857] - (0.1ug/ml) staining of Rat Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry: Ephrin-B2 Antibody [NBP1-49857]

Immunohistochemistry: Ephrin-B2 Antibody [NBP1-49857]

Ephrin-B2-Antibody-Immunohistochemistry-NBP1-49857-img0002.jpg

Applications for Ephrin-B2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 35 kDa band observed in human, mouse and rat lung lysates (calculated MW of 36.9 kDa band according to NP_004084.1). Use in IHC-P reported in scientific literature (PMID: 29133876).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ephrin-B2

This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors.

Alternate Names

EFNB2, ELF-2, EphrinB2, Htk-L, LERK-5, NLERK-1

Entrez Gene IDs

1948 (Human); 13642 (Mouse); 306636 (Rat)

Gene Symbol

EFNB2

UniProt

P52799

Additional Ephrin-B2 Products

Product Documents for Ephrin-B2 Antibody

Certificate of Analysis

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Product Specific Notices for Ephrin-B2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Ephrin-B2 Antibody

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Protocols

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