EPHX1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33011

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Epoxide Hydrolase 1/EPHX1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human EPHX1. The exact sequence is proprietary.

Localization

Microsome membrane, Single-pass membrane protein, Endoplasmic reticulum membrane, Single-pass membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EPHX1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: EPHX1 Antibody [NBP1-33011]

Immunocytochemistry/ Immunofluorescence: EPHX1 Antibody [NBP1-33011]

Immunocytochemistry/Immunofluorescence: EPHX1 Antibody [NBP1-33011] - Analysis of methanol-fixed HeLa, using EPHX1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: EPHX1 Antibody [NBP1-33011]

Immunohistochemistry-Paraffin: EPHX1 Antibody [NBP1-33011]

Immunohistochemistry-Paraffin: EPHX1 Antibody [NBP1-33011] - Mouse liver. EPHX1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: EPHX1 Antibody [NBP1-33011]

Immunohistochemistry-Paraffin: EPHX1 Antibody [NBP1-33011]

Immunohistochemistry-Paraffin: EPHX1 Antibody [NBP1-33011] - SG xenograft, using Epoxide hydrolase 1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
EPHX1 Antibody - BSA Free

Western Blot: EPHX1 Antibody [NBP1-33011] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with EPHX1 antibody (NBP1-33011) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
EPHX1 Antibody - BSA Free

Western Blot: EPHX1 Antibody [NBP1-33011] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with EPHX1 antibody (NBP1-33011) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for EPHX1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Epoxide Hydrolase 1/EPHX1

Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Alternate Names

EPHX, EPHX1, EPOX, Epoxide Hydratase, HYL1, MEH, MEH8, Microsomal Epoxide Hydrolase

Gene Symbol

EPHX1

Additional Epoxide Hydrolase 1/EPHX1 Products

Product Documents for EPHX1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for EPHX1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Redox Enzymes

Citations for EPHX1 Antibody - BSA Free

Customer Reviews for EPHX1 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review EPHX1 Antibody - BSA Free and earn rewards!

Have you used EPHX1 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies