EPHX2 Antibody (OTI1A6)

Novus Biologicals | Catalog # NBP2-02948

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A6
View all Epoxide Hydrolase 2/EPHX2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human EPHX2 (NP_001970) produced in HEK293T cell.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31002701). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

62.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EPHX2 Antibody (OTI1A6)

Western Blot: EPHX2 Antibody (1A6) [NBP2-02948] Analysis of extracts (35ug) from 9 different cell lines by using anti-EPHX2 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: EPHX2 Antibody (OTI1A6) [NBP2-02948]

Immunocytochemistry/ Immunofluorescence: EPHX2 Antibody (OTI1A6) [NBP2-02948]

Immunocytochemistry/Immunofluorescence: EPHX2 Antibody (1A6) [NBP2-02948] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY EPHX2.
Immunohistochemistry-Paraffin: EPHX2 Antibody (OTI1A6) [NBP2-02948]

Immunohistochemistry-Paraffin: EPHX2 Antibody (OTI1A6) [NBP2-02948]

Immunohistochemistry-Paraffin: EPHX2 Antibody (1A6) [NBP2-02948] - Staining of paraffin-embedded Human prostate tissue using anti-EPHX2 mouse monoclonal antibody.
Western Blot: EPHX2 Antibody (OTI1A6) [NBP2-02948]

Western Blot: EPHX2 Antibody (OTI1A6) [NBP2-02948]

Western Blot: EPHX2 Antibody (1A6) [NBP2-02948] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHX2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHX2.
Immunohistochemistry-Paraffin: EPHX2 Antibody (OTI1A6) [NBP2-02948]

Immunohistochemistry-Paraffin: EPHX2 Antibody (OTI1A6) [NBP2-02948]

Immunohistochemistry-Paraffin: EPHX2 Antibody (1A6) [NBP2-02948] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-EPHX2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: EPHX2 Antibody (OTI1A6) [NBP2-02948]

Immunohistochemistry-Paraffin: EPHX2 Antibody (OTI1A6) [NBP2-02948]

Immunohistochemistry-Paraffin: EPHX2 Antibody (1A6) [NBP2-02948] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-EPHX2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: EPHX2 Antibody (OTI1A6) [NBP2-02948]

Immunohistochemistry-Paraffin: EPHX2 Antibody (OTI1A6) [NBP2-02948]

Immunohistochemistry-Paraffin: EPHX2 Antibody (1A6) [NBP2-02948] - Staining of paraffin-embedded Human endometrium tissue using anti-EPHX2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: EPHX2 Antibody (OTI1A6) [NBP2-02948]

Immunohistochemistry-Paraffin: EPHX2 Antibody (OTI1A6) [NBP2-02948]

Immunohistochemistry-Paraffin: EPHX2 Antibody (1A6) [NBP2-02948] - Staining of paraffin-embedded Human Kidney tissue using anti-EPHX2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: EPHX2 Antibody (OTI1A6) [NBP2-02948]

Immunohistochemistry-Paraffin: EPHX2 Antibody (OTI1A6) [NBP2-02948]

Immunohistochemistry-Paraffin: EPHX2 Antibody (1A6) [NBP2-02948] - Staining of paraffin-embedded Human liver tissue using anti-EPHX2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: EPHX2 Antibody (OTI1A6) [NBP2-02948]

Immunohistochemistry-Paraffin: EPHX2 Antibody (OTI1A6) [NBP2-02948]

Immunohistochemistry-Paraffin: EPHX2 Antibody (1A6) [NBP2-02948] - Staining of paraffin-embedded Human lymphoma tissue using anti-EPHX2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: EPHX2 Antibody (OTI1A6) [NBP2-02948]

Immunohistochemistry-Paraffin: EPHX2 Antibody (OTI1A6) [NBP2-02948]

Immunohistochemistry-Paraffin: EPHX2 Antibody (1A6) [NBP2-02948] - Staining of paraffin-embedded Human pancreas tissue using anti-EPHX2 mouse monoclonal antibody.
EPHX2 Antibody (OTI1A6) Western Blot Shows EPHX2 Specificity Using Knockout Cell Line.

Western Blot Shows EPHX2 Specificity Using Knockout Cell Line.

Western blot shows lysates of K562 human chronic myelogenous leukemia parental cell line and EPHX2 knockout K562 cell line (KO). Nitrocellulose membrane was probed with EPHX2 Antibody (OTI1A6) (Catalog # NBP2-02948) followed by HRP-conjugated secondary antibody. A specific band was detected for EPHX2 at approximately 62.6 kDa (as indicated) in the parental K562 cell line, but is not detectable in knockout K562 cell line. Primary antibody dilution used: 1/500. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Applications for EPHX2 Antibody (OTI1A6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Knockout Validated

Validated for Knockout from YCharOS Inc. (YCharOS.com)

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.7 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Epoxide Hydrolase 2/EPHX2

This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes, binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq]. This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes, binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq].

Alternate Names

Bifunctional Epoxide Hydrolase 2, CEH, EPHX2, Epoxide Hydratase 2, Epoxide Hydrolase 2, Cytosolic, SEH

Entrez Gene IDs

2053 (Human)

Gene Symbol

EPHX2

Additional Epoxide Hydrolase 2/EPHX2 Products

Product Documents for EPHX2 Antibody (OTI1A6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for EPHX2 Antibody (OTI1A6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Redox Enzymes

Citations for EPHX2 Antibody (OTI1A6)

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Protocols

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