epithelial Sodium Channel gamma Antibody (3c7) - BSA Free

Novus Biologicals | Catalog # NBP2-41371

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Sandwich ELISA, Sandwich ELISA Capture

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3c7

Format

BSA Free
View all epithelial Sodium Channel gamma Primary Antibodies »

Product Specifications

Immunogen

The inhibitory peptide from the human gamma-ENaC subunit. EAESWNSVSEGKQPRFSHRIPLC corresponding to amino acid residue 139-160 of human gamma-ENaC subunit.

Epitope

The epitope is on the inhibitory tract of human ENaC.

Specificity

Antibody is specific for the inhibitory tract of human gamma-ENaC subunit.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for epithelial Sodium Channel gamma Antibody (3c7) - BSA Free

Western Blot: epithelial Sodium Channel gamma Antibody (3c7)BSA Free [NBP2-41371]

Western Blot: epithelial Sodium Channel gamma Antibody (3c7)BSA Free [NBP2-41371]

Western Blot: epithelial Sodium Channel gamma Antibody (3c7) - BSA Free [NBP2-41371] - Analysis on a lysate of HEK293 cells transfected with and without yENaC. Lane 1 loaded with 5ug Lane 2 loaded with 10ug Lane 3 loaded with 20ug
ELISA: epithelial Sodium Channel gamma Antibody (3c7) - BSA Free [NBP2-41371]

ELISA: epithelial Sodium Channel gamma Antibody (3c7) - BSA Free [NBP2-41371]

ELISA: epithelial Sodium Channel gamma Antibody (3c7) - BSA Free [NBP2-41371] - The calibration curve of a sandwich assay for Anti-gENaC using NBP2-41371 as the capture antibody and NBP2-41373B as the biotinylated detection antibody.

Applications for epithelial Sodium Channel gamma Antibody (3c7) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Sandwich ELISA Capture

1-4 ug/ml

Western Blot

1:100-1:2000
Application Notes
ELISA: NBP2-41371 was used in ELISA. A sandwich ELISA can be made using NBP2-41371 (1-4 ug/ml) as the capture antibody and biotinylated NBP2-41373 (0.05 - 0.2 ug/ml) as the detection antibody in order to detect the peptide from the inhibitory tract (AA 138-131). WB: NBP2-41371 was used in Western blot. (1,2) IHC: NBP2-41371 was used in immunohistochemistry.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: epithelial Sodium Channel gamma

Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the gamma subunit, and mutations in this gene have been associated with Liddle syndrome. [provided by RefSeq]

Long Name

Amiloride-sensitive sodium channel subunit gamma

Alternate Names

ENaCG, Gamma-ENaC, Gamma-NaCH, SCNEG, SCNN1G

Gene Symbol

SCNN1G

Additional epithelial Sodium Channel gamma Products

Product Documents for epithelial Sodium Channel gamma Antibody (3c7) - BSA Free

Certificate of Analysis

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Product Specific Notices for epithelial Sodium Channel gamma Antibody (3c7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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