EPLIN Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-87947
![Immunocytochemistry/ Immunofluorescence: EPLIN Antibody [NBP1-87947]](https://resources.rndsystems.com/images/products/EPLIN-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87947-img0016.jpg "Immunocytochemistry/ Immunofluorescence: EPLIN Antibody [NBP1-87947]")
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Key Product Details
Validated by
Orthogonal Validation, Independent Antibodies
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ISENSYSLDDLEIGPGQLSSSTFDSEKNESRRNLELPRLSETSIKDRMAKYQAAVSKQSSSTNYTNELKASGGEIKIHKMEQKENVPPGPEVCITHQEGEKISANENSLAVRSTPAEDDSRDS
Reactivity Notes
Mouse reactivity reported in the scientific literature (PMID: 24135282).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit EPLIN Antibody - BSA Free (NBP1-87947) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-EPLIN Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for EPLIN Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: EPLIN Antibody [NBP1-87947]
Immunocytochemistry/Immunofluorescence: EPLIN Antibody [NBP1-87947] - Staining of human cell line U-2 OS shows localization to plasma membrane, cytosol and actin filaments. Antibody staining is shown in green.![Western Blot: EPLIN Antibody [NBP1-87947]](https://resources.rndsystems.com/images/products/EPLIN-Antibody-Western-Blot-NBP1-87947-img0018.jpg "Western Blot: EPLIN Antibody [NBP1-87947]")
![EPLIN Antibody - BSA Free Western Blot: EPLIN Antibody - BSA Free [NBP1-87947]](https://resources.rndsystems.com/images/products/nbp1-87947_rabbit-polyclonal-eplin-antibody-652025162165.jpg "Western Blot: EPLIN Antibody - BSA Free [NBP1-87947]")
![EPLIN Antibody - BSA Free Immunohistochemistry-Paraffin: EPLIN Antibody - BSA Free [NBP1-87947]](https://resources.rndsystems.com/images/products/nbp1-87947_rabbit-polyclonal-eplin-antibody-6520251625132.jpg "Immunohistochemistry-Paraffin: EPLIN Antibody - BSA Free [NBP1-87947]")
![EPLIN Antibody - BSA Free Immunohistochemistry-Paraffin: EPLIN Antibody - BSA Free [NBP1-87947]](https://resources.rndsystems.com/images/products/nbp1-87947_rabbit-polyclonal-eplin-antibody-6520251622277.jpg "Immunohistochemistry-Paraffin: EPLIN Antibody - BSA Free [NBP1-87947]")
![EPLIN Antibody - BSA Free Immunohistochemistry-Paraffin: EPLIN Antibody - BSA Free [NBP1-87947]](https://resources.rndsystems.com/images/products/nbp1-87947_rabbit-polyclonal-eplin-antibody-6520251619423.jpg "Immunohistochemistry-Paraffin: EPLIN Antibody - BSA Free [NBP1-87947]")
Immunohistochemistry-Paraffin: EPLIN Antibody - BSA Free [NBP1-87947]
Staining of human rectum shows strong membranous and cytoplasmic positivity in glandular cells.![EPLIN Antibody - BSA Free Immunohistochemistry-Paraffin: EPLIN Antibody - BSA Free [NBP1-87947]](https://resources.rndsystems.com/images/products/nbp1-87947_rabbit-polyclonal-eplin-antibody-65202516264324.jpg "Immunohistochemistry-Paraffin: EPLIN Antibody - BSA Free [NBP1-87947]")
Immunohistochemistry-Paraffin: EPLIN Antibody - BSA Free [NBP1-87947]
Staining of human liver shows very weak membranous positivity in hepatocytes.![EPLIN Antibody - BSA Free Immunohistochemistry-Paraffin: EPLIN Antibody - BSA Free [NBP1-87947]](https://resources.rndsystems.com/images/products/nbp1-87947_rabbit-polyclonal-eplin-antibody-6520251625282.jpg "Immunohistochemistry-Paraffin: EPLIN Antibody - BSA Free [NBP1-87947]")
Immunohistochemistry-Paraffin: EPLIN Antibody - BSA Free [NBP1-87947]
Staining of human prostate shows strong membranous positivity in glandular cells.![EPLIN Antibody - BSA Free Immunohistochemistry-Paraffin: EPLIN Antibody - BSA Free [NBP1-87947]](https://resources.rndsystems.com/images/products/nbp1-87947_rabbit-polyclonal-eplin-antibody-65202516175019.jpg "Immunohistochemistry-Paraffin: EPLIN Antibody - BSA Free [NBP1-87947]")
Immunohistochemistry-Paraffin: EPLIN Antibody - BSA Free [NBP1-87947]
Staining of human skin shows moderate membranous and cytoplasmic positivity in squamous epithelial cells.Applications for EPLIN Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval method is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: EPLIN
Alternate Names
1110021C24Rik, Epithelial protein lost in neoplasm, EPLINepithelial protein lost in neoplasm beta, FLJ38853, LIM domain and actin binding 1, MGC131726, SREBP3LIM domain and actin-binding protein 1, sterol regulatory element binding protein 3
Entrez Gene IDs
51474 (Human)
Gene Symbol
LIMA1
UniProt
Additional EPLIN Products
Product Documents for EPLIN Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for EPLIN Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for EPLIN Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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