EPS8L2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83613

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EPS8L2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SVSCPLLSRDAVDFLRGHLVPKEMSLWESLGESWMRPRSEWPREPQVPLYVPKFHSGWEPPVDVLQEAPWEVEGLASAPIEEVSPVSR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EPS8L2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: EPS8L2 Antibody [NBP1-83613]

Immunocytochemistry/ Immunofluorescence: EPS8L2 Antibody [NBP1-83613]

Immunocytochemistry/Immunofluorescence: EPS8L2 Antibody [NBP1-83613] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: EPS8L2 Antibody [NBP1-83613]

Immunohistochemistry-Paraffin: EPS8L2 Antibody [NBP1-83613]

Immunohistochemistry-Paraffin: EPS8L2 Antibody [NBP1-83613] - Staining in human small intestine and skeletal muscle tissues.. Corresponding EPS8L2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: EPS8L2 Antibody [NBP1-83613]

Immunohistochemistry-Paraffin: EPS8L2 Antibody [NBP1-83613]

Immunohistochemistry-Paraffin: EPS8L2 Antibody [NBP1-83613] - Staining of human skin shows strong cytoplasmic positivity in epidermal cells.
Immunohistochemistry-Paraffin: EPS8L2 Antibody [NBP1-83613]

Immunohistochemistry-Paraffin: EPS8L2 Antibody [NBP1-83613]

Immunohistochemistry-Paraffin: EPS8L2 Antibody [NBP1-83613] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: EPS8L2 Antibody [NBP1-83613]

Immunohistochemistry-Paraffin: EPS8L2 Antibody [NBP1-83613]

Immunohistochemistry-Paraffin: EPS8L2 Antibody [NBP1-83613] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
EPS8L2 Antibody - BSA Free Immunohistochemistry-Paraffin: EPS8L2 Antibody - BSA Free [NBP1-83613]

Immunohistochemistry-Paraffin: EPS8L2 Antibody - BSA Free [NBP1-83613]

Staining of human skeletal muscle shows no positivity in myocytes.
EPS8L2 Antibody - BSA Free Western Blot: EPS8L2 Antibody - BSA Free [NBP1-83613]

Western Blot: EPS8L2 Antibody - BSA Free [NBP1-83613]

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
EPS8L2 Antibody - BSA Free Western Blot: EPS8L2 Antibody - BSA Free [NBP1-83613]

Western Blot: EPS8L2 Antibody - BSA Free [NBP1-83613]

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells))
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells))

Applications for EPS8L2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EPS8L2

EPS8L2 encodes a member of the EPS8 gene family. The encoded protein, like other members of the family, is thought to link growth factor stimulation to actin organization, generating functional redundancy in the pathways that regulate actin cytoskeletal remodeling. [provided by RefSeq]

Alternate Names

epidermal growth factor receptor kinase substrate 8-like protein 2, Epidermal growth factor receptor pathway substrate 8-related protein 2, EPS8-like 2, EPS8-like protein 2, EPS8R2, EPS8-related protein 2, FLJ16738, FLJ21935, FLJ22171, MGC126530, MGC3088

Gene Symbol

EPS8L2

Additional EPS8L2 Products

Product Documents for EPS8L2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for EPS8L2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for EPS8L2 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review EPS8L2 Antibody - BSA Free and earn rewards!

Have you used EPS8L2 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies