ER alpha/NR3A1 Antibody (6A6E8) - BSA Free

Novus Biologicals | Catalog # NBP2-61764

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6A6E8

Format

BSA Free
View all ER alpha/NR3A1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human ER alpha/NR3A1 (AA: 2-185) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

66.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ER alpha/NR3A1 Antibody (6A6E8) - BSA Free

Western Blot: ER alpha/NR3A1 Antibody (6A6E8)BSA Free [NBP2-61764]

Western Blot: ER alpha/NR3A1 Antibody (6A6E8)BSA Free [NBP2-61764]

Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mAb against HEK293 (1) and ESR1 (AA: 2-185)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: ER alpha/NR3A1 Antibody (6A6E8) - BSA Free [NBP2-61764]

Immunocytochemistry/ Immunofluorescence: ER alpha/NR3A1 Antibody (6A6E8) - BSA Free [NBP2-61764]

Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of Hela cells using ESR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (6A6E8) - BSA Free [NBP2-61764]

Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (6A6E8) - BSA Free [NBP2-61764]

Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of esophageal cancer tissues using ESR1 mouse mAb with DAB staining.
Flow Cytometry: ER alpha/NR3A1 Antibody (6A6E8) - BSA Free [NBP2-61764]

Flow Cytometry: ER alpha/NR3A1 Antibody (6A6E8) - BSA Free [NBP2-61764]

Flow Cytometry: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of Hela cells using ESR1 mouse mAb (green) and negative control (red).
Western Blot: ER alpha/NR3A1 Antibody (6A6E8)BSA Free [NBP2-61764]

Western Blot: ER alpha/NR3A1 Antibody (6A6E8)BSA Free [NBP2-61764]

Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mAb against human ESR1 (AA: 2-185) recombinant protein. (Expected MW is 45.8 kDa)
Western Blot: ER alpha/NR3A1 Antibody (6A6E8)BSA Free [NBP2-61764]

Western Blot: ER alpha/NR3A1 Antibody (6A6E8)BSA Free [NBP2-61764]

Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mouse mAb against MOLT4 (1), Raji (2), MCF-7 (3), T47D (4), SK-Br-3 (5), Hela (6), and C6 (7) cell lysate.
Immunocytochemistry/ Immunofluorescence: ER alpha/NR3A1 Antibody (6A6E8) - BSA Free [NBP2-61764]

Immunocytochemistry/ Immunofluorescence: ER alpha/NR3A1 Antibody (6A6E8) - BSA Free [NBP2-61764]

Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of Hela cells using ESR1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (6A6E8) - BSA Free [NBP2-61764]

Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (6A6E8) - BSA Free [NBP2-61764]

Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of breast cancer tissues using ESR1 mouse mAb with DAB staining.
ELISA: ER alpha/NR3A1 Antibody (6A6E8) - BSA Free [NBP2-61764]

ELISA: ER alpha/NR3A1 Antibody (6A6E8) - BSA Free [NBP2-61764]

ELISA: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for ER alpha/NR3A1 Antibody (6A6E8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1-1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ER alpha/NR3A1

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined.;

Long Name

Estrogen Receptor alpha

Alternate Names

ESR1, NR3A1

Gene Symbol

ESR1

Additional ER alpha/NR3A1 Products

Product Documents for ER alpha/NR3A1 Antibody (6A6E8) - BSA Free

Certificate of Analysis

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Product Specific Notices for ER alpha/NR3A1 Antibody (6A6E8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ER alpha/NR3A1 Antibody (6A6E8) - BSA Free

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Protocols

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FAQs

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail