ER alpha/NR3A1 Antibody (CL1196) - BSA Free

Novus Biologicals | Catalog # NBP2-34478

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1196

Format

BSA Free
View all ER alpha/NR3A1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P03372, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ER alpha/NR3A1 Antibody (CL1196) - BSA Free

Western Blot: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478]

Western Blot: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478]

Western Blot: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ESR1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478]

Immunocytochemistry/ Immunofluorescence: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478]

Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of MCF7 cells using the Anti-ESR1 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478]

Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478]

Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human testis shows absence of immunoreactivity (negative control).
Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478]

Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478]

Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human endometrium shows strong nuclear immunoreactivity in glandular cells.
Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478]

Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478]

Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human cervix shows strong nuclear positivity in epithelial cells.
Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478]

Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478]

Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human fallopian tube shows strong immunoreactivity in epithelial cells.
Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478]

Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478]

Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human breast cancer shows moderate to strong nuclear positivity in a subset of tumor cells.

Applications for ER alpha/NR3A1 Antibody (CL1196) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ER alpha/NR3A1

Estrogen receptor alpha (ER alpha) is a nuclear protein and member of the steroid hormone receptor family. ER alpha possesses both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes (1). Ligand-dependent dimerization and phosphorylation on serines, 104, 106, and 118 function to regulate the transcriptional activation of ER alpha (2). The phosphorylation of the human estrogen receptor (ER) serine residue at position 118 is required for full activity of the ER activation function 1 (AF-1) (3).

Long Name

Estrogen Receptor alpha

Alternate Names

ESR1, NR3A1

Gene Symbol

ESR1

UniProt

P03372

Additional ER alpha/NR3A1 Products

Product Documents for ER alpha/NR3A1 Antibody (CL1196) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ER alpha/NR3A1 Antibody (CL1196) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for ER alpha/NR3A1 Antibody (CL1196) - BSA Free

There are currently no reviews for this product. Be the first to review ER alpha/NR3A1 Antibody (CL1196) - BSA Free and earn rewards!

Have you used ER alpha/NR3A1 Antibody (CL1196) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies

Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail