Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 002 expressed in HEK293

Format

Azide and BSA Free
View all ErbB2/Her2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human ErbB2/Her2 cytoplasmic domain (Accession#: NP_004439.2; Lys 676-Val 1255).

Specificity

No cross-reactivity in ELISA with: Human ErbB1 / EGFRHuman ErbB3 / HER3Human ErbB4 / HER4

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ErbB2/Her2 Antibody (002) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (002) [NBP2-89184]

Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (002) [NBP2-89184]

Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (002) [NBP2-89184] - Staining of Human ErbB2 in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human ErbB2 monoclonal antibody (1:100) at 4c overnight. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody ( green ) and counterstained with DAPI ( blue ). Positive staining was localized to plasma membrane.
Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (002) [NBP2-89184]

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (002) [NBP2-89184]

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (002) [NBP2-89184] - Staining of human ERBB2 in human breast carcinoma with rabbit monoclonal antibody (1:200).

Applications for ErbB2/Her2 Antibody (002) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:50-1:1000

Immunohistochemistry-Paraffin

1:100-1:500

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ErbB2/Her2

ErbB2/HER2 is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases (1). The kinase activity of ErbB2 can be activated without ligand if it is overexpressed, and by association with other ErbB proteins (2). Overexpression of ErbB2 is detected in almost 40% of human breast cancers (3). Binding of c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to poly-ubiquitination of ErbB2 and enhances its degradation (4). ErbB2 is one of the major targets for the treatment of breast cancer and other carcinomas.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Alternate Names

CD340, HER2, Neu Oncogene, NGL, TKR1

Gene Symbol

ERBB2

Additional ErbB2/Her2 Products

Product Documents for ErbB2/Her2 Antibody (002) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ErbB2/Her2 Antibody (002) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ErbB2/Her2 Antibody (002) - Azide and BSA Free

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    • Q: I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?
      A: A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22
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