ErbB2/Her2 Antibody (HRB2/451)

Novus Biologicals | Catalog # NBP2-29437

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Flow Cytometry, Flow (Cell Surface), Immunofluorescence, Immunocytochemistry/ Immunofluorescence, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # HRB2/451
View all ErbB2/Her2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human ErbB2/Her2 protein (Uniprot: IDP04626)

Localization

Cell Surface

Specificity

This monoclonal antibody is specific to c-erbB-2/HER-2 and shows minimal cross-reaction with other members of the family. C-erbB-2/HER-2 is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

185 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33064)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for ErbB2/Her2 Antibody (HRB2/451)

Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]

Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]

Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437] - ICC/IF staining of MCF-7 cells with followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Red Dot (Red).
Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]

Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]

Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437] - Flow Cytometric Analysis of human trypsinized MCF-7 cells. ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/451) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]

Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]

Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437] - Immunofluorescent staining of PFA-fixed MCF-7 cells with ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/451) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).
Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]

Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]

Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437] - Flow Cytometric Analysis of human trypsinized SK-BR3 cells. ErbB2/Her2 Antibody (HRB2/451) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
Protein Array: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]

Protein Array: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]

Protein Array: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ErbB2/Her2 Antibody (HRB2/451) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD?s) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD?s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for ErbB2/Her2 Antibody (HRB2/451)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-4 ug/ml

Immunofluorescence

1-2ug/ml

Immunohistochemistry-Paraffin

0.5-1.0ug/ml
Application Notes
ELISA: For coating, order antibody without BSA)
Optimal dilution for a specific application should be determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: ErbB2/Her2

Also known as erbB2 and CD340, HER-2 is closely related in structure to the epidermal growth factor receptor. It is over-expressed in a variety of carcinomas, especially those of breast and ovary.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Alternate Names

CD340, HER2, Neu Oncogene, NGL, TKR1

Gene Symbol

ERBB2

UniProt

P04626

Additional ErbB2/Her2 Products

Product Documents for ErbB2/Her2 Antibody (HRB2/451)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ErbB2/Her2 Antibody (HRB2/451)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ErbB2/Her2 Antibody (HRB2/451)

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    • Q: I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?
      A: A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22
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