ErbB2/Her2 Antibody (OTI4F10)

Novus Biologicals | Catalog # NBP2-01152

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Whole-Mount, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry Whole-Mount

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI4F10
View all ErbB2/Her2 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 676-1255 of human ERBB2(NP_004439) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

Antibody specificy evaluation by using microarray chip and WB analysis showed that this antibody cross-reacts with CDH1 recombinant protein in overexpression lysates.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

137.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse ErbB2/Her2 Antibody (OTI4F10) (NBP2-01152) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-ErbB2/Her2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ErbB2/Her2 Antibody (OTI4F10)

Western Blot: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]

Western Blot: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]

Western Blot: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152] - Analysis of extracts (35ug) from 2 different Human cell lines, dilution (1:500).
Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]

Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]

Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ErbB2/HER2.
Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]

Immunohistochemistry-Paraffin: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - Staining of paraffin-embedded Human Kidney tissue using anti-ErbB2/HER2 mouse monoclonal antibody.
Flow Cytometry: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]

Flow Cytometry: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]

Flow Cytometry: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ErbB2/HER2 antibody, and then analyzed by flow cytometry.
Western Blot: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]

Western Blot: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]

Western Blot: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ErbB2/HER2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ErbB2/HER2.
Western Blot: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]

Western Blot: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]

Western Blot: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - 1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8:Colon.
Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]

Immunohistochemistry-Paraffin: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ErbB2/HER2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]

Immunohistochemistry-Paraffin: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ErbB2/HER2 mouse monoclonal antibody.

Applications for ErbB2/Her2 Antibody (OTI4F10)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry Whole-Mount

Reported in scientific literature (PMID:34944780)

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ErbB2/Her2

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq].

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Alternate Names

CD340, HER2, Neu Oncogene, NGL, TKR1

Entrez Gene IDs

2064 (Human); 2064 (Human)

Gene Symbol

ERBB2

Additional ErbB2/Her2 Products

Product Documents for ErbB2/Her2 Antibody (OTI4F10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ErbB2/Her2 Antibody (OTI4F10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ErbB2/Her2 Antibody (OTI4F10)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ErbB2/Her2 Antibody (OTI4F10)

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    • Q: I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?
      A: A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22
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