Q: I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?

A: A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22

ErbB2/Her2 Antibody

Novus Biologicals | Catalog # NB100-1710

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 975 and 1025 of human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 using the numbering given in entry NP_004439.2 (GeneID 2064).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit ErbB2/Her2 Antibody (NB100-1710) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-ErbB2/Her2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ErbB2/Her2 Antibody

Western Blot: ErbB2/Her2 Antibody [NB100-1710]

Western Blot: ErbB2/Her2 Antibody [NB100-1710] - Detection of Human ErbB2 on HeLa whole cell lysate using NB100-1710. ErbB2 was also immunoprecipitated using rabbit anti-ErbB2 antibodies NB100-1711 and NB100-1712.

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NB100-1710]

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NB100-1710] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ErbB2 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for ErbB2/Her2 Antibody

Application

Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

1-4 ug/mg of lysate

Western Blot

1:2000-1:10000

Application Notes

Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ErbB2/Her2

ErbB2 is a member of the receptor tyrosine kinase family of proteins. The four members of this family are ErbB1 (Epidermal growth factor receptor; EGFR/HER1), ErbB2 (HER2/Neu), ErbB3 (HER3), and ErbB4 (HER4). The ErbB receptor members are single-pass transmembrane proteins with an extracellular ligand-binding domain and a cytoplasmic tyrosine-kinase domain. The epidermal growth factor (EGF) and its related proteins serve as the ligands for the ErbB receptors. Upon binding, the receptors heterodimerize, autophosphorylate and make contact with adapter molecules to transmit cellular signals involved in cell proliferation, survival, and differentiation. The ErbB2 receptor is the only member that does not interact with a ligand, but instead functions as a heterodimeric partner to coordinate signals from other ligand bound ErbB receptors. Amplification and/or overexpression of ErbB2 have been reported in many cancers. Alternate names for ErbB2 include receptor tyrosine-protein kinase erbB-2. p185erbB2, C-erbB-2, Neu proto-oncogene, tyrosine kinase-type cell surface receptor HER2, TKR1, c-erb B2, MLN 19, ERBB2, HER2, NEU, and NGL.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Alternate Names

CD340, HER2, Neu Oncogene, NGL, TKR1

Entrez Gene IDs

2064 (Human); 2064 (Human)

Gene Symbol

ERBB2

UniProt

NP_004439.2 (Human)

Additional ErbB2/Her2 Products

Product Documents for ErbB2/Her2 Antibody

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Product Specific Notices for ErbB2/Her2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Cancer Stem Cell Markers

EGF Family

Receptor Tyrosine Kinases (RTKs)

Citations for ErbB2/Her2 Antibody

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