ErbB2/Her2 [p Tyr877] Antibody - BSA Free
Novus Biologicals | Catalog # NB100-81961
![Western Blot: ErbB2/Her2 [p Tyr877] Antibody [NB100-81961]](https://beta-resources.rndsystems.com/images/products/ErbB2-Her2-[p-Tyr877]-Antibody-Western-Blot-NB100-81961-img0001.jpg "Western Blot: ErbB2/Her2 [p Tyr877] Antibody [NB100-81961]")
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human ErbB2/Her2 around the phosphorylation site of tyrosine 877 (T-E-YP-H-A).
Modification
p Tyr877
Specificity
Detects endogenous levels of ErbB2/Her2 only when phosphorylated at tyrosine 877.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit ErbB2/Her2 [p Tyr877] Antibody - BSA Free (NB100-81961) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-ErbB2/Her2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for ErbB2/Her2 [p Tyr877] Antibody - BSA Free
![ErbB2/Her2 [p Tyr877] Antibody - BSA Free](https://beta-resources.rndsystems.com/images/products/nb100-81961_rabbit-polyclonal-erbb2-her2-p-tyr877-antibody-113202594525.jpg "Immunohistochemistry-Paraffin: ErbB2/Her2 [p Tyr877] Antibody [NB100-81961] -")
Immunohistochemistry-Paraffin: ErbB2/Her2 [p Tyr877] Antibody [NB100-81961] -
Immunohistochemistry-Paraffin: ErbB2/Her2 [p Tyr877] Antibody [NB100-81961] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81961 incubated with blocking peptide (right)![ErbB2/Her2 [p Tyr877] Antibody - BSA Free](https://beta-resources.rndsystems.com/images/products/nb100-81961_rabbit-polyclonal-erbb2-her2-p-tyr877-antibody-113202515145437.jpg "Immunocytochemistry/Immunofluorescence: ErbB2/Her2 [p Tyr877] Antibody [NB100-81961] -")
Immunocytochemistry/Immunofluorescence: ErbB2/Her2 [p Tyr877] Antibody [NB100-81961] -
Immunocytochemistry/Immunofluorescence: ErbB2/Her2 [p Tyr877] Antibody [NB100-81961] - Staining of methanol-fixed MCF7 cells using NB100-81961Applications for ErbB2/Her2 [p Tyr877] Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:200
Immunohistochemistry
1:50-1:100
Immunohistochemistry-Paraffin
1:50-1:100
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ErbB2/Her2
Long Name
Receptor Tyrosine Protein Kinase ErbB2
Alternate Names
CD340, HER2, Neu Oncogene, NGL, TKR1
Gene Symbol
ERBB2
UniProt
Additional ErbB2/Her2 Products
Product Documents for ErbB2/Her2 [p Tyr877] Antibody - BSA Free
Product Specific Notices for ErbB2/Her2 [p Tyr877] Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for ErbB2/Her2 [p Tyr877] Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for ErbB2/Her2 [p Tyr877] Antibody - BSA Free
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Q: I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?
A: A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22
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Associated Pathways
MAPK Signaling Pathway: Mitogen Stimulation Pathway
mTOR Signaling Pathway
