Erbin Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-13968

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Erbin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: WHSKQNPQIDHASFPPQLLPRSESTENQSYAKHSANMNFSNHNNVRANTAYHLHQRLGPARHGEMWAISPNDRLIPAVTRSTIQR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (84%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Erbin Antibody - BSA Free (NBP2-13968) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Erbin Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Erbin Antibody - BSA Free

Western Blot: Erbin Antibody [NBP2-13968]

Western Blot: Erbin Antibody [NBP2-13968]

Western Blot: Erbin Antibody [NBP2-13968] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ERBIN antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: Erbin Antibody [NBP2-13968]

Immunocytochemistry/ Immunofluorescence: Erbin Antibody [NBP2-13968]

Immunocytochemistry/Immunofluorescence: Erbin Antibody [NBP2-13968] - Staining of human cell line A-431 shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Erbin Antibody [NBP2-13968]

Immunohistochemistry-Paraffin: Erbin Antibody [NBP2-13968]

Immunohistochemistry-Paraffin: Erbin Antibody [NBP2-13968] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Erbin Antibody [NBP2-13968]

Immunohistochemistry-Paraffin: Erbin Antibody [NBP2-13968]

Immunohistochemistry-Paraffin: Erbin Antibody [NBP2-13968] - Staining of human breast shows strong membranous positivity in myoepithelial cells.
Immunohistochemistry-Paraffin: Erbin Antibody [NBP2-13968]

Immunohistochemistry-Paraffin: Erbin Antibody [NBP2-13968]

Immunohistochemistry-Paraffin: Erbin Antibody [NBP2-13968] - Staining of human gastrointestinal shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Erbin Antibody [NBP2-13968]

Immunohistochemistry-Paraffin: Erbin Antibody [NBP2-13968]

Immunohistochemistry-Paraffin: Erbin Antibody [NBP2-13968] - Staining of human salivary gland shows moderate membranous positivity in glandular cells.

Applications for Erbin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Erbin

The ERBB2IP gene encodes a LAP2 protein that exists in nine isoforms: Isoform 1: 1,412 amino acids long, 158 kDA; isoform 2: 1,371 amino acids long, 153 kDA; isoform 3: 1,360 amino acids long, 152 kDA; isoform 4: 1,346 amino acids, 151 kDA; isoform 5: 1,356 amino acids long, 152 kDA; isoform 6: 1,340 amino acids long, 150 kDA; isoform 7: 1,302 amino acids long, 146 kDA; isoform 8: 1,419 amino acids long, 159 kDA; isoform 9 is 1,367 amino acids long at 153 kDA. ERBB2IP functions as an adapter for the receptor ERBB2, and through binding of this receptor, it contributes to the stabilization of this unphosphorylated state. ERBB2IP participates in the NOD pathway, TGF-beta receptor signaling pathway, signal transduction, and with NLR proteins. It is known to interact with genes PKP4, SMAD3, ERBB2, ZFYVE9, and CTNNB1. ERBB2IP is associated with breast cancer, carcinoma, cervical cancer, crohn's disease, and epidermolysis bullosa.

Long Name

ErbB2-interacting Protein

Alternate Names

Densin-180-like protein, ERBB2IP

Gene Symbol

ERBIN

Additional Erbin Products

Product Documents for Erbin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Erbin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

EGF Family
NFkB Pathway

Citations for Erbin Antibody - BSA Free

Customer Reviews for Erbin Antibody - BSA Free

There are currently no reviews for this product. Be the first to review Erbin Antibody - BSA Free and earn rewards!

Have you used Erbin Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies