ERCC1 Antibody (CL1284) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-43960

Novus Biologicals

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1284

Format

Azide and BSA Free
View all ERCC1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P07992, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse ERCC1 Antibody (CL1284) - Azide and BSA Free (NBP2-34480) is a monoclonal antibody validated for use in IHC, WB and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ERCC1 Antibody (CL1284) - Azide and BSA Free

ERCC1 Antibody (CL1284) - Azide and BSA Free Immunohistochemistry-Paraffin: ERCC1 Antibody [NBP3-43960]

Immunohistochemistry-Paraffin: ERCC1 Antibody [NBP3-43960]

Staining of human breast cancer shows moderate nuclear positivity in tumor cells.
ERCC1 Antibody (CL1284) - Azide and BSA Free Immunohistochemistry-Paraffin: ERCC1 Antibody [NBP3-43960]

Immunohistochemistry-Paraffin: ERCC1 Antibody [NBP3-43960]

Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.
ERCC1 Antibody (CL1284) - Azide and BSA Free Immunohistochemistry-Paraffin: ERCC1 Antibody [NBP3-43960]

Immunohistochemistry-Paraffin: ERCC1 Antibody [NBP3-43960]

Staining of human prostate cancer shows moderate to strong nuclear positivity in tumor cells.
ERCC1 Antibody (CL1284) - Azide and BSA Free Western Blot: ERCC1 Antibody [NBP3-43960]

Western Blot: ERCC1 Antibody [NBP3-43960]

Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ERCC1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
ERCC1 Antibody (CL1284) - Azide and BSA Free Immunohistochemistry-Paraffin: ERCC1 Antibody [NBP3-43960]

Immunohistochemistry-Paraffin: ERCC1 Antibody [NBP3-43960]

Staining of human tonsil shows moderate nuclear positivity in lymphoid cells.
ERCC1 Antibody (CL1284) - Azide and BSA Free Immunohistochemistry-Paraffin: ERCC1 Antibody [NBP3-43960]

Immunohistochemistry-Paraffin: ERCC1 Antibody [NBP3-43960]

Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Applications for ERCC1 Antibody (CL1284) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200 - 1:500

Simple Western

1:20

Western Blot

1 ug/ml
Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. See Simple Western Antibody Database for Simple Western validation: Tested in RT4, separated by Size, antibody dilution of 1:20, apparent MW was 46 kDa

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: ERCC1

The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1 XPF complex makes the 5'incision. Elevated levels of ERCC1 have also been reported in Cisplatin resistant cells.

Long Name

Excision Repair Cross-complementing 1

Alternate Names

COFS4, DNA excision repair protein ERCC-1, excision repair cross-complementing rodent repair deficiency, complementationgroup 1 (includes overlapping antisense sequence), RAD10, UV20

Gene Symbol

ERCC1

Additional ERCC1 Products

Product Documents for ERCC1 Antibody (CL1284) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ERCC1 Antibody (CL1284) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Customer Reviews for ERCC1 Antibody (CL1284) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review ERCC1 Antibody (CL1284) - Azide and BSA Free and earn rewards!

Have you used ERCC1 Antibody (CL1284) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies