ERR alpha/NR3B1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-47254

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Canine (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ERR alpha/NR3B1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an N-terminal portion of human Estrogen Related Receptor alpha (within residues 1-50). [Swiss-Prot# P11474]

Reactivity Notes

Predicted to react with rat, pig and dog based on 100% sequence homology.

Localization

Nucleus, Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ERR alpha/NR3B1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ERR alpha/NR3B1 Antibody - BSA Free [NBP1-47254]

Immunocytochemistry/ Immunofluorescence: ERR alpha/NR3B1 Antibody - BSA Free [NBP1-47254]

Immunocytochemistry/Immunofluorescence: ERR alpha/NR3B1 Antibody [NBP1-47254] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with conjugated to DyLight 488 (NBP1-47254G) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Western Blot: ERR alpha/NR3B1 AntibodyBSA Free [NBP1-47254]

Western Blot: ERR alpha/NR3B1 AntibodyBSA Free [NBP1-47254]

ERR-alpha-NR3B1-Antibody-Western-Blot-NBP1-47254-img0011.jpg
Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody - BSA Free [NBP1-47254]

Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody - BSA Free [NBP1-47254]

Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP1-47254] - IHC-P detection of ERR alpha/ESRRA protein in a section of normal human kidney using 5 ug/ml concentration of Estrogen Related Receptor alpha antibody. Some cells of tubules/ducts in the tested renal tissue depicted intense nuclear staining with an overall weak/diffused cytoplasmic staining. (10X Magnification)
Western Blot: ERR alpha/NR3B1 AntibodyBSA Free [NBP1-47254]

Western Blot: ERR alpha/NR3B1 AntibodyBSA Free [NBP1-47254]

Western Blot: ERR alpha/NR3B1 Antibody [NBP1-47254] - Analysis of ERR alpha in A431 cell lysates
Immunocytochemistry/ Immunofluorescence: ERR alpha/NR3B1 Antibody - BSA Free [NBP1-47254]

Immunocytochemistry/ Immunofluorescence: ERR alpha/NR3B1 Antibody - BSA Free [NBP1-47254]

Immunocytochemistry/Immunofluorescence: ERR alpha/NR3B1 Antibody [NBP1-47254] - ERR alpha antibody was tested in HeLa cells at a 1:100 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.
Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody - BSA Free [NBP1-47254]

Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody - BSA Free [NBP1-47254]

Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP1-47254] - IHC-P detection of ERR alpha / ESRRA protein in a section of normal skin of human using 5 ug/ml concentration of Estrogen Related Receptor alpha antibody. The different cell types in the cellular epidermis as well as the blood vessels in the dermal layer showed positivity for ERR alpha, and the staining was found to be more intense in the nuclei compared to cytoplasm. (10X Magnification)

Applications for ERR alpha/NR3B1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

2 ug/ml

Immunohistochemistry-Paraffin

2 ug/ml

Western Blot

2 ug/ml
Application Notes
This Estrogen Related Receptor alpha antibody is useful for Western Blot, Immunohistochemistry-Paraffin and Immunocytochemistry/Immunofluorescence applications. ERR alpha/ ESRRA localize mainly to the nucleus, but there are reports on its cytoplasmic presence also, especially in cells with high nuclear expression. In a feedback loop mechanism, it has been shown that ERK8 /MAPK15 interacts with ERR alpha to induce its CRM1-dependent translocation to cytoplasm for inhibiting its transcriptional activity via regulation of its cellular localization (PMIDs 21190936, 18070081). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 3 using NBP1-47254 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERR alpha/NR3B1

Nuclear receptors are essential in embryonic development as well as for the regulation of adult physiological functions, and estrogen related receptor alpha (ERR-alpha) is one such receptors expressed in several tissues requiring high energy demand, including heart, skeletal muscle and brain. ERR-alpha binds to ERRE (ERR-alpha response element) containing a single consensus half-site, 5'-TNAAGGTCA-3'; to MCAD response element NRRE-1 for regulating MCAD promoter, and it also regulates lactoferrin gene promoter. ERR-alpha is an orphan nuclear hormone receptor capable of regulating transcription of genes involved in multiple cellular/physiological processes such as regulation of energy metabolism wherein it is co-activated by PPAR gamma and PGC-1 alpha to regulate several genes required for mitochondrial function. ERR-alpha regulates estrogen signaling and fatty acid oxidation/adaptative bioenergetic response, and is involved in cartilage/bone formation, macrophage activation and carcinogenesis. High ERR-alpha expression levels coupled with poor prognosis have been observed in various malignancies including colon, endometrium, ovary, prostate and breast cancers, and adrenocortical carcinoma.

Long Name

Estrogen-related Receptor alpha

Alternate Names

ERR1, ESRL1, ESRRA, NR3B1

Entrez Gene IDs

2101 (Human); 26379 (Mouse); 293701 (Rat)

Gene Symbol

ESRRA

UniProt

P11474 (Human)

Additional ERR alpha/NR3B1 Products

Product Documents for ERR alpha/NR3B1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ERR alpha/NR3B1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ERR alpha/NR3B1 Antibody - BSA Free

Customer Reviews for ERR alpha/NR3B1 Antibody - BSA Free (1)

3 out of 5
1 Customer Rating
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  • ERR alpha/NR3B1 Antibody - BSA Free
    Name: Anonymous
    Application: Western Blot
    Sample Tested: glioblastoma cell lysates
    Species: Human
    Verified Customer | Posted 08/02/2023
    human glioblastoma cell lysate. 10 ug loaded. condition: control, drug1, 2, and 3 treatment left numbers: size marker. bands of 50kDa is true band. There are multi bands.
    ERR alpha/NR3B1 Antibody - BSA Free NBP1-47254

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Protocols

View specific protocols for ERR alpha/NR3B1 Antibody - BSA Free (NBP1-47254):

ERR alpha/NR3B1 Antibody:
Western Blot Protocol
1. Perform SDS-PAGE (4-12% MOPS) on samples to be analyzed, loading 35 ug of total protein per lane.
2. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
3. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
4. Rinse the blot in TBS for approximately 5 minutes.
5. Block the membrane using 5% NFDM + 1% BSA in TBS + Tween, 1 hour at RT.
6. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.
7. Dilute the rabbit anti-ERR alpha primary antibody (NBP1-45282) in blocking buffer and incubate 1 hour at room temperature.
8. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.
9. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).
Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%, provided it does not interfere with antibody-antigen binding.

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